2013 Promega catalogue

Life
Science
Catalog
2013
Worldwide Contact List
Cell Signaling
Sample Preparation for
Genetic Identity
SwabSolution Kit, PunchSolution Kit and
5X AmpSolution Reagent
Product Size Cat.# Price ($)
SwabSolution Kit 100 preps DC8271 300
PunchSolution Kit 100 preps DC9271 240
5X AmpSolution Reagent 500 μl DM1231 195
Not For Medical Diagnostic Use.
Description: The SwabSolution Kit, PunchSolution Kit and 5X
AmpSolution Reagent allow fast and simple processing of swabs and
punches for PowerPlex ® System analysis. These products are intended for
preparation of single-source reference, database and paternity samples where
sample purification is unnecessary.
The SwabSolution Kit is used for rapid processing of swabs for STR analysis
using PowerPlex ® Systems. The SwabSolution Kit contains SwabSolution
Reagent, which is used to generate a buccal swab extract that is added to the
PowerPlex ® System reaction. In addition, the SwabSolution Kit contains a 5X
AmpSolution Reagent, which enables direct amplification from swabs with
PowerPlex ® Systems that were not originally designed for direct amplification.
See the supported PowerPlex ® Systems at: www.promega.com/directamp/
The PunchSolution Kit is used for rapid processing of punches from
nonFTA storage cards (S&S 903, Bode Buccal Collector device, etc.) for
STR analysis using PowerPlex ® Systems. The PunchSolution Kit contains
PunchSolution Reagent, which is used to pretreat nonFTA punches prior to
addition of the PowerPlex ® PCR amplification mix. In addition, the
PunchSolution Kit contains the 5X AmpSolution Reagent, which enables
direct amplification from punches with PowerPlex ® Systems that were not
originally designed for direct amplification. See the supported PowerPlex ®
Systems at: www.promega.com/directamp/
The 5X AmpSolution Reagent allows direct amplification of unwashed
FTA ® punches in most PowerPlex ® Systems that were not originally designed
for direct amplification. Additionally, the AmpSolution Reagent allows use of
the SwabSolution and PunchSolution Kits with more PowerPlex ® Systems
(5X AmpSolution Reagent is included in the SwabSolution and
PunchSolution Kits). The AmpSolution Reagent is simply added to a
PowerPlex ® PCR amplification mix. See the supported PowerPlex ® Systems at:
www.promega.com/directamp/
Features:
• Rapid, simple preparation methods for swabs and punches can save
2–4 hours per plate of samples.
• Compatibility with most PowerPlex ® Systems increases the speed and
versatility of the PowerPlex ® Systems.
Storage Conditions: Upon receipt of kit, thaw and mix by gentle inversion as
described in the technical manual, and store at 4°C.
Protocol
SwabSolution Kit Technical Manual
PunchSolution Kit Technical Manual
Place punch
card into well.
Add 10µl of
PunchSolution
Reagent.
Incubate for 30 minutes
at 70°C (to dryness).
Part#
TMD037
TMD038
Add PCR
amplification mix.
Perform thermal
cycling.
PunchSolution Kit nonFTA punch workflow. For systems other than
PowerPlex ® 18D, PowerPlex ® 21, PowerPlex ® Y23 and PowerPlex ® Fusion
Systems, add AmpSolution Reagent to the PCR amplification mix. For
more information, please visit: www.promega.com/directamp/
Add buccal swab
to tube or plate*.
Add 1ml of
SwabSolution
Reagent.
Incubate for
30 minutes at
70°C**.
Transfer 2µl of
swab extract.
Add to 23µl of PCR
amplification mix.
Perform thermal
cycling.
SwabSolution Kit buccal swab workflow. For systems other than
PowerPlex ® 18D, PowerPlex ® 21, PowerPlex ® Y23 and PowerPlex ® Fusion
Systems, add AmpSolution Reagent. For more information, please
visit: www.promega.com/directamp/
*For the plate format, use a 2.2ml, Square-Well Deep Well Plate (Cat.#
V6781).
**Use Heat Block Adapter (Cat.# A2661), with the heat set at 90°C.
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Section
Contents
Table of
Contents
178
For complete and up-to-date product information visit: www.promega.com/catalog