2013 Promega catalogue

Life
Science
Catalog
2013
Worldwide Contact List
Cell Signaling
HaloLink Protein Array System
Product Size Cat.# Price ($)
HaloLink Array Six Slide System 6 slides G6190 Pls. Enq.
HaloTag ® Standard Protein 30 μg G4491 91
Protein G HaloTag ® Fusion Protein 5 mg G7291 500
For Research Use Only. Not for Use in Diagnostic Procedures.
For additional information see page 285.
Magne HaloTag ® Beads
Product Size Cat.# Price ($)
Magne HaloTag ® Beads, 20% Slurry 1 ml G7281 192
For Research Use Only. Not for Use in Diagnostic Procedures.
For additional information see page 285.
HaloCHIP System
5 ml G7282 765
Product Size Cat.# Price ($)
HaloCHIP System 20 reactions G9410 846
For Research Use Only. Not for Use in Diagnostic Procedures.
For additional information see page 286.
HaloLink Resin
Product Size Cat.# Price ($)
HaloLink Resin 1.25 ml G1912 520
For Research Use Only. Not for Use in Diagnostic Procedures.
For additional information see page 284.
2.5 ml G1913 206
10 ml G1914 474
25 ml G1915 867
HaloTag ® Mammalian Pull-Down Systems
Product Size Cat.# Price ($)
HaloTag ® Complete Pull-Down System 1 each G6509 1270
HaloTag ® Mammalian Pull-Down and Labeling
System
24 reactions G6500 532
HaloTag ® Mammalian Pull-Down System 24 reactions G6504 290
HaloTag ® Control Vector 20 μg G6591 236
Available Separately Size Cat.# Price ($)
Protease Inhibitor Cocktail, 50X 1 ml G6521 89
Mammalian Lysis Buffer 40 ml G9381 60
For Research Use Only. Not for Use in Diagnostic Procedures.
For additional information see page 283.
MagneGST Pull-Down System
Product Size Cat.# Price ($)
MagneGST Pull-Down System 80 reactions V8870 913
For Research Use Only. Not for Use in Diagnostic Procedures.
For additional information see page 288.
Gel Shift Assay Systems
Product Size Cat.# Price ($)
Gel Shift Assay Core System 100 reactions E3050 604
Gel Shift Assay System 100 reactions E3300 783
Available Separately Size Cat.# Price ($)
HeLaScribe ® Nuclear Extract, Gel Shift Assay
Grade 3 × 40 μl E3521 324
Gel Shift Binding 5X Buffer 5 × 200 μl E3581 497
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The gel shift or electrophoretic mobility shift assay provides a
simple and rapid method for detecting DNA-binding proteins. This method
is widely used to study sequence-specific DNA-binding proteins such as
transcription factors. The assay is based on the observation that complexes
of protein and DNA migrate through a nondenaturing polyacrylamide gel more
slowly than free DNA fragments or double-stranded oligonucleotides. The gel
shift assay is performed by incubating a purified protein or a complex mixture
of proteins (such as nuclear or cell extract preparations) with a 32 P end-labeled
DNA fragment containing the putative protein binding site. The reaction products
are then analyzed on a nondenaturing polyacrylamide gel. The specificity of the
DNA-binding protein for the putative binding site is established by competition
experiments using unlabeled DNA fragments or oligonucleotides containing a
binding site for the protein of interest or other unrelated DNA sequences.
The Core System (Cat.# E3050) includes HeLa Nuclear Extract and SP1 and
AP2 Consensus Oligos that can be used as positive controls and serve as a
reliable system for obtaining experience with gel shift assays. In addition, the
Core System contains T4 Polynucleotide Kinase and Kinase 10X Buffer for
labeling oligonucleotides as well as Gel Shift Binding 5X Buffer. Cat.# E3300
contains all of the above plus consensus oligos for AP1, OCT1, CREB, NF-κB,
and TFIID.
Features:
• Positive Controls: The Gel Shift Assay Core System includes a HeLa
Nuclear Extract and consensus oligonucleotides for AP2 and SP1.
• Versatile: Oligonucleotides can be 5´ end-labeled and used as proteinspecific
probes or used as unlabeled oligonucleotides in competition
assays.
Storage Conditions: Store HeLa Nuclear Extract at –70°C. Store other
components at –20°C.
Protocol
Gel Shift Assay System Technical Bulletin
Part#
TB110
Section
Contents
Table of
Contents
292
For complete and up-to-date product information visit: www.promega.com/catalog