2013 Promega catalogue

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Life Science Catalog <strong>2013</strong> Worldwide Contact List Cell Signaling HaloLink Protein Array System Product Size Cat.# Price ($) HaloLink Array Six Slide System 6 slides G6190 Pls. Enq. HaloTag ® Standard Protein 30 μg G4491 91 Protein G HaloTag ® Fusion Protein 5 mg G7291 500 For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 285. Magne HaloTag ® Beads Product Size Cat.# Price ($) Magne HaloTag ® Beads, 20% Slurry 1 ml G7281 192 For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 285. HaloCHIP System 5 ml G7282 765 Product Size Cat.# Price ($) HaloCHIP System 20 reactions G9410 846 For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 286. HaloLink Resin Product Size Cat.# Price ($) HaloLink Resin 1.25 ml G1912 520 For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 284. 2.5 ml G1913 206 10 ml G1914 474 25 ml G1915 867 HaloTag ® Mammalian Pull-Down Systems Product Size Cat.# Price ($) HaloTag ® Complete Pull-Down System 1 each G6509 1270 HaloTag ® Mammalian Pull-Down and Labeling System 24 reactions G6500 532 HaloTag ® Mammalian Pull-Down System 24 reactions G6504 290 HaloTag ® Control Vector 20 μg G6591 236 Available Separately Size Cat.# Price ($) Protease Inhibitor Cocktail, 50X 1 ml G6521 89 Mammalian Lysis Buffer 40 ml G9381 60 For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 283. MagneGST Pull-Down System Product Size Cat.# Price ($) MagneGST Pull-Down System 80 reactions V8870 913 For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 288. Gel Shift Assay Systems Product Size Cat.# Price ($) Gel Shift Assay Core System 100 reactions E3050 604 Gel Shift Assay System 100 reactions E3300 783 Available Separately Size Cat.# Price ($) HeLaScribe ® Nuclear Extract, Gel Shift Assay Grade 3 × 40 μl E3521 324 Gel Shift Binding 5X Buffer 5 × 200 μl E3581 497 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The gel shift or electrophoretic mobility shift assay provides a simple and rapid method for detecting DNA-binding proteins. This method is widely used to study sequence-specific DNA-binding proteins such as transcription factors. The assay is based on the observation that complexes of protein and DNA migrate through a nondenaturing polyacrylamide gel more slowly than free DNA fragments or double-stranded oligonucleotides. The gel shift assay is performed by incubating a purified protein or a complex mixture of proteins (such as nuclear or cell extract preparations) with a 32 P end-labeled DNA fragment containing the putative protein binding site. The reaction products are then analyzed on a nondenaturing polyacrylamide gel. The specificity of the DNA-binding protein for the putative binding site is established by competition experiments using unlabeled DNA fragments or oligonucleotides containing a binding site for the protein of interest or other unrelated DNA sequences. The Core System (Cat.# E3050) includes HeLa Nuclear Extract and SP1 and AP2 Consensus Oligos that can be used as positive controls and serve as a reliable system for obtaining experience with gel shift assays. In addition, the Core System contains T4 Polynucleotide Kinase and Kinase 10X Buffer for labeling oligonucleotides as well as Gel Shift Binding 5X Buffer. Cat.# E3300 contains all of the above plus consensus oligos for AP1, OCT1, CREB, NF-κB, and TFIID. Features: • Positive Controls: The Gel Shift Assay Core System includes a HeLa Nuclear Extract and consensus oligonucleotides for AP2 and SP1. • Versatile: Oligonucleotides can be 5´ end-labeled and used as proteinspecific probes or used as unlabeled oligonucleotides in competition assays. Storage Conditions: Store HeLa Nuclear Extract at –70°C. Store other components at –20°C. Protocol Gel Shift Assay System Technical Bulletin Part# TB110 Section Contents Table of Contents 292 For complete and up-to-date product information visit: www.promega.com/catalog
Cell Signaling Protein Purification Accessories Magnetic Stands and Spacers Product Size Cat.# Price ($) MagnaBot ® 96 Magnetic Separation Device 1 each V8151 813 MagnaBot ® II Magnetic Separation Device 1 each V8351 Pls. Enq. MagnaBot ® Flat Top Magnetic Separation Device 1 each V6041 Pls. Enq. Plate Clamp 96 1 each V8251 Pls. Enq. Plate Stand 1 each V8261 Pls. Enq. Deep Well MagnaBot ® 96 Magnetic Separation Device 1 each V3031 Pls. Enq. Heat Transfer Block 1 each Z3271 98 Heat Block Insert 1 each Z3651 174 MagnaBot ® Spacer 3/16 inch 1 each V8381 Pls. Enq. MagnaBot ® Spacer 1/8 inch 1 each V8581 Pls. Enq. MagnaBot ® Spacer 1/16 inch 1 each V8681 Pls. Enq. 1/4 inch Foam Spacer 1 each Z3301 36 MagnaBot ® 384 Magnetic Separation Device 1 each V8241 Pls. Enq. 384-Well Plate, Flat 10 /pk V5291 Pls. Enq. 384-Well Plate, Conical 10 /pk V5311 Pls. Enq. V8151, V8351, V6041, V8251, V8261, Z3271, V8381, V8581, V8681, Z3301, V5291, V5311 For Research Use Only. Not for Use in Diagnostic Procedures. V3031, Z3651, V8241 For Laboratory Use. MagnaBot ® II Magnetic Separation Device (Cat.# V8351). Plate Clamp 96 (Cat.# V8251) with a 96-well PCR plate. 3417TA05_1A 16 Protein Purification and Interactions MagnaBot ® 96 Magnetic Separation Device (Cat.# V8151). 3375TA05_1A 3993TA02_3A Plate Stand (Cat.# V8261). 3373TA05_1A 2889TA03_0A MagnaBot ® 96 Magnetic Separation Device (Cat.# V8151) with a 96-well Collection Plate and robotic gripper arm. MagnaBot ® 384 Magnetic Separation Device (Cat.# V8241). 3995TA02_3A Section Contents For complete and up-to-date product information visit: www.promega.com/catalog 293 Table of Contents
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Life Science CATALOG 2013 Table of
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Cell Signaling Table of Contents Pr
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Cell Signaling 1 Biobanking 1 DNA E
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Cell Signaling DNA Extraction for B
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Cell Signaling Wizard ® Genomic DN
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Cell Signaling QuantiFluor RNA Syst
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Cell Signaling Sample ID and Mixed
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Cell Signaling 2 Biochemicals and L
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Cell Signaling Agarose, LMP, Prepar
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Cell Signaling Blue/Orange Loading
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Cell Signaling Glycine, Molecular B
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Cell Signaling SDS Solution, Molecu
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Cell Signaling Tris Base, Molecular
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Cell Signaling Unmethylated Lambda
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Cell Signaling Promega Barrier Tips
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Cell Signaling Magnetic Stands and
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Cell Signaling 3 Cell Health Assays
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Cell Signaling A. B. Luminescence/p
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Cell Signaling Luminogenic Enzyme S
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Cell Signaling Apoptosis
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Cell Signaling Caspase-Glo ® 2 Ass
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Cell Signaling Caspase-Glo ® 8 Ass
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Cell Signaling CaspACE Assay System
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Cell Signaling Anti-ACTIVE ® Caspa
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Cell Signaling CellTiter-Glo ® One
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Cell Signaling Fluorescent Cell Via
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Cell Signaling CellTiter-Blue ® Ce
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Cell Signaling ApoTox-Glo Triplex A
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Cell Signaling CytoTox-Fluor Cytoto
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Cell Signaling GSH-Glo Glutathione
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Cell Signaling 4 Cell Signaling 4 G
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Cell Signaling cAMP-Glo Max Assay P
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Cell Signaling Growth Factors Epide
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Cell Signaling SIRT-Glo Assays and
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Cell Signaling ADP-Glo Kinase Assay
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Cell Signaling Kinase Enzyme System
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Cell Signaling ProFluor ® PKA Assa
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Cell Signaling PepTag ® Non-Radioa
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Cell Signaling Product Size Cat.# P
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Cell Signaling kDa 98 - 64 - 50 - 3
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Cell Signaling ProFluor ® Ser/Thr
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Cell Signaling 5 Cloning and DNA Ma
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Cell Signaling DNA Step Ladders Pro
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Cell Signaling Conventional DNA Mar
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Cell Signaling RNA Markers Product
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Cell Signaling AatII 37 Product Siz
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Cell Signaling BstEII 60 Product Si
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Cell Signaling HhaI 37 Product Size
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Cell Signaling NdeI 37 Product Size
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Cell Signaling SnaBI 37 Product Siz
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Cell Signaling Alkaline Phosphatase
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Cell Signaling T4 DNA Polymerase Pr
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Cell Signaling Kinases and DNA Labe
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Cell Signaling Additional Enzymes S
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Cell Signaling Subcloning Tools and
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Cell Signaling pALTER ® -MAX Vecto
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Cell Signaling pGEM ® -7Zf(+/-) Ve
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Cell Signaling pSP72 Vector Product
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Cell Signaling 6 DNA and RNA Purifi
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Cell Signaling x-tracta Gel Extract
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Cell Signaling Wizard ® MagneSil
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Cell Signaling ReliaPrep Blood gDNA
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Cell Signaling Wizard ® SV 96 Geno
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Cell Signaling Fixed-Tissue Genomic
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Cell Signaling Maxwell ® 16 System
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Cell Signaling PureYield Plasmid Mi
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Cell Signaling Wizard ® Plus Minip
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Cell Signaling Wizard ® SV 96 and
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Cell Signaling RNA Purification Rel
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Cell Signaling PureYield RNA Midipr
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Cell Signaling RNasin ® Plus RNase
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Cell Signaling DNA and RNA Quantita
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Cell Signaling Magnetic Stands and
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Cell Signaling Vacuum Manifolds and
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Cell Signaling 7 Drug Discovery 7 D
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Cell Signaling GPCR Assays cAMP-Glo
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Cell Signaling GloResponse Lucifera
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Cell Signaling DPPIV-Glo Protease A
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Cell Signaling Calpain-Glo Protease
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Cell Signaling 8 Epigenetics 8 DNA
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Cell Signaling DNA Purification Tec
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Cell Signaling Luciferase-Based Met
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Cell Signaling GoTaq ® Long PCR Ma
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Cell Signaling DUB-Glo Protease Ass
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Cell Signaling HaloTag ® Protein P
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Cell Signaling 9 Genetic Identity 9
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Cell Signaling DNA IQ System Produc
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Cell Signaling DNA IQ Casework Pro
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Cell Signaling STR Analysis for For
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Cell Signaling PowerPlex ® Y23 Sys
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Cell Signaling PowerPlex ® 18D Sys
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Cell Signaling PowerPlex ® S5 Syst
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Cell Signaling GenePrint ® Fluores
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Cell Signaling 10 Imaging and Immun
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling NGF E max ® ImmunoA
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Cell Signaling Anti-ACTIVE ® CaM K
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Cell Signaling A. Anti-ACTIVE ® MA
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Cell Signaling Anti-ACTIVE ® Caspa
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Cell Signaling Anti-NGF mAb Product
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Cell Signaling Anti-TGFβ 1 pAb Pro
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Cell Signaling Horseradish Peroxida
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Cell Signaling ViviRen In Vivo Reni
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Cell Signaling 11 Industrial and En
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Cell Signaling QuantiLum ® Recombi
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12 Instruments 12 Luminometers 218
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GloMax ® 20/20 Luminometer Product
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Fluorescence Module: Application-op
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GloMax ® -Multi Jr Single-Tube Mul
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Maxwell ® CSC System for IVD Use M
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Maxwell ® Research Systems Maxwell
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Maxwell ® 16 Flexi Method Firmware
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Cell Signaling 13 Molecular Diagnos
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Cell Signaling Maxwell ® CSC Servi
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Cell Signaling Maxwell ® 16 Flexi
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Cell Signaling Deoxynucleotide Trip
Page 245 and 246: Cell Signaling 14 PCR 14 Hot-Start
Page 247 and 248: Cell Signaling Routine PCR GoTaq ®
Page 249 and 250: Cell Signaling Pfu DNA Polymerase d
Page 251 and 252: Cell Signaling GoTaq ® Real-Time q
Page 253 and 254: Cell Signaling StemElite Human Panc
Page 255 and 256: Cell Signaling Reverse Transcriptio
Page 257 and 258: Cell Signaling AMV Reverse Transcri
Page 259 and 260: Cell Signaling PCR Cloning pGEM ®
Page 261 and 262: Cell Signaling 15 Protein Expressio
Page 263 and 264: Cell Signaling TnT ® Quick Coupled
Page 265 and 266: Cell Signaling TnT ® T7 Quick for
Page 267 and 268: Cell Signaling Canine Pancreatic Mi
Page 269 and 270: Cell Signaling E. coli S30 Extract
Page 271 and 272: Cell Signaling Mass Spectrometry An
Page 273 and 274: Cell Signaling Endoproteinase Lys-C
Page 275 and 276: Cell Signaling Protein Labeling and
Page 277 and 278: Cell Signaling HaloTag ® Fusion (C
Page 279 and 280: Cell Signaling Inducible T7-Driven
Page 281 and 282: Cell Signaling ECL Western Blotting
Page 283 and 284: Cell Signaling 16 Protein Purificat
Page 285 and 286: Cell Signaling HaloTag ® Protein P
Page 287 and 288: HT Cell Signaling HaloTag ® Mammal
Page 289 and 290: Cell Signaling HaloLink Protein Arr
Page 291 and 292: Cell Signaling FastBreak Cell Lysis
Page 293 and 294: Cell Signaling MagneHis Protein Pur
Page 295: Cell Signaling Streptavidin Product
Page 299 and 300: Cell Signaling 17 Reporter Assays a
Page 301 and 302: Cell Signaling NanoLuc Luciferase
Page 303 and 304: Cell Signaling Chroma-Glo Luciferas
Page 305 and 306: Cell Signaling ONE-Glo + Tox Lucife
Page 307 and 308: Cell Signaling Luciferase Assay Sys
Page 309 and 310: Cell Signaling ViviRen Live Cell Su
Page 311 and 312: Cell Signaling Reporter Vectors and
Page 313 and 314: Cell Signaling Promoterless Renilla
Page 315 and 316: Cell Signaling pmirGLO Dual-Lucifer
Page 317 and 318: Cell Signaling pGL2 Luciferase Repo
Page 319 and 320: Cell Signaling Reporter Vector Sequ
Page 321 and 322: Cell Signaling pCAT ® 3 Vectors Pr
Page 323 and 324: Cell Signaling ViviRen In Vivo Reni
Page 325 and 326: Cell Signaling 18 RNA Analysis 18 I
Page 327 and 328: Cell Signaling Riboprobe ® System
Page 329 and 330: Cell Signaling HeLaScribe ® Nuclea
Page 331 and 332: Cell Signaling RNA Interference Gen
Page 333 and 334: Cell Signaling 19 Stem Cell Researc
Page 335 and 336: Cell Signaling Cell ID System Fluor
Page 337 and 338: Cell Signaling StemElite Human Panc
Page 339 and 340: Cell Signaling 20 STR Analysis 20 C
Page 341 and 342: Cell Signaling PowerPlex ® 16 HS S
Page 343 and 344: Cell Signaling StemElite ID System
Page 345 and 346: Cell Signaling 21 Vectors 21 Bacter
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Cell Signaling Mammalian Expression
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Cell Signaling pCI-neo Mammalian Ex
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Cell Signaling pSV-β-Galactosidase
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22 Index 22 Index: A-Z 354 Index by
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Index: A-Z Click on name to go to p
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Index by Catalog Number Click on pa
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1S11 TH01 D3S1358 FGA TPOX D8S1179
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2013 Promega catalogue

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