2013 Promega catalogue

Life
Science
Catalog
2013
Worldwide Contact List
Cell Signaling
Systems for Molecular Diagnostics
Research
Microsatellite Instability (MSI) Analysis
Product Size Cat.# Price ($)
MSI Analysis System, Version 1.2 100 reactions MD1641 Pls. Enq.
Available Separately Size Cat.# Price ($)
Internal Lane Standard 600 150 μl DG1071 362
DG1071 For Laboratory Use. MD1641 For Research Use Only. Not for Use in Diagnostic
Procedures.
Description: The MSI Analysis System, Version 1.2, is a fluorescent multiplex
PCR-based method detect microsatellite instability (MSI), a form of genomic
instability. This instability is due to insertion or deletion of repeating units
during DNA replication and failure of the mismatch repair system (MMR) to
correct these errors. MSI analysis typically involves comparing allelic profiles
of microsatellite markers generated by amplification from matching pairs of
test samples, which may be MMR-deficient, and normal tissue samples. New
alleles in the abnormal sample not found in the corresponding normal sample
indicate the presence of MSI. MSI analysis can be used as a screening method
to identify samples for further characterization.
The MSI Analysis System, Version 1.2, includes fluorescently labeled primers
(marker panel) for co-amplification of seven markers for analysis of the MSIhigh
(MSI-H) phenotype, including five nearly monomorphic mononucleotide
repeat markers (BAT-25, BAT-26, MON0-27, NR-21 and NR-24) and two highly
polymorphic pentanucleotide repeat markers (Penta C and Penta D). Amplified
fragments are detected using an ABI PRISM ® 310, 3100, 3100-Avant, 3130 or
3130xl Genetic Analyzer after spectral calibration.
Panels and bins text files simplify and standardize data analysis by providing
automated assignment of genotypes using GeneMapper ® 4.0 software.
Features:
• Understand the Complete MSI Phenotype: A single multiplex PCR
amplifies five informative mononucleotide repeat markers for MSI-H determination.
• Confidence in Sample Identification: Co-amplification of highly polymorphic
pentanucleotide repeats provides internal sample tracking.
• Consistent Data Analysis: MSI Panels and bins for GeneMapper ® 4.0
software can be downloaded.
Storage Conditions: Store at –20°C.
Protocol
MSI Analysis System, Version 1.2 Technical Manual
A.
B.
C.
D.
Part#
TM255
Y Chromosome Deletion Detection System,
Version 2.0
Product Size Cat.# Price ($)
Y Chromosome Deletion Detection System, 25 reactions MD1531 Pls. Enq.
Version 2.0
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Y Chromosome Deletion Detection System, Version 2.0,
provides a standardized screening panel amplifying only informative nonpolymorphic
sequence tag sites (STS) on the human Y chromosome. The system
amplifies key functional regions associated with AZoospermia Factor (AZF),
namely the regions that flank AZFa and cover AZFb, AZFc, AZFd including DAZ,
KAL-Y, SMCY and flanking loci for other key spermatogenesis-related genes
(namely RBM1, DFFRY and DBY).
Five Multiplex Master Mixes, with a total of 20 characterized Y-specific primer
pairs, are included. Four of the multiplex primer sets contain a control primer
pair that amplifies a fragment of the X-linked SMCX locus. One of the multiplex
primer sets (Multiplex E Master Mix) contains a control primer pair that amplifies
a unique region in both male and female DNA (ZFX/ZFY). Finally, a primer
pair that amplifies a region of the SRY gene has been included in Multiplex E
Master Mix as a control for the testis-determining factor on the short arm of the
Y chromosome to detect XX males arising from Y to X translocations.
The Multiplex Master Mixes are designed to facilitate the simultaneous amplification
of several different regions of the Y chromosome. The amplification
products (83–496bp) of the five multiplex PCR amplifications can be clearly
separated by agarose gel electrophoresis and visualized by ethidium bromide
staining.
Failure to amplify specific regions of the Y chromosome is indicative of Y
chromosome deletions in the test sample. The size control ladder provided
minimizes analysis time and the possibility of misinterpreting molecular weight
of amplification products.
Features:
• Ease of Use: Premixed Multiplex Master Mixes contain 20 primer pairs,
including internal controls providing a standardized panel of results requiring
no user optimization.
• More Robust Reactions: Improved formulation and use of GoTaq ® DNA
Polymerase minimizes dropouts.
• Flexibility: Amplify genomic DNA purified using various methods and with
a PE480 (oil overlay) or PE9600/9700 (non-oil overlay) thermal cycler.
• Complete System: All required reagents are provided in the kit.
Storage Conditions: Store at –20°C.
Protocol
Y Chromosome Deletion Detection System, Version 2.0 Technical
Manual
Primer Sets in the Y Chromosome Deletion Detection System.
Part#
TM248
Locus/ Locus/ Locus/ Locus/ Locus/
Multiplex STS 1 STS 2 STS 3 STS 4 STS 5
Master Mix A DAZ/ DYS240/ DYS271/ DYS221/ KAL-Y/
SY254 SY157 SY81 SY130 SY182
Master Mix B SMCY/ DYS218/ DAZ/ DAZ/
SYPR3 SY127 SY242 SY208
Master Mix C DYS219/ DYS212/ DYF51S1/ DAZ/
SY128 SY121 SY145 SY255
Master Mix D DYS236/ DYS223/ DYS215/
SY152 SY133 SY124
Master Mix E SRY/ DYS224/ DYS148/ DYS273/ ZFX1/
SY14 SY134 SY86 SY84 ZFY
9492LA
Section
Contents
Analysis of MSI phenotype.
4586TA
Table of
Contents
240
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