2013 Promega catalogue

Life
Science
Catalog
2013
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Cell Signaling
Steady-Glo ® Luciferase Assay System
Product Size Cat.# Price ($)
Steady-Glo ® Luciferase Assay System 10 ml E2510 187
For Research Use Only. Not for Use in Diagnostic Procedures.
100 ml E2520 1371
10 × 100 ml E2550 Pls. Enq.
Description: High-throughput quantitation of firefly (Photinus pyralis) luciferase
expression in mammalian cells is commonly performed by batch processing
of 96- and 384-well plates. Steady-Glo ® Luciferase Assay System is designed
for this purpose by providing long-lived luminescence when added to cultured
cells. The homogeneous assay provides signal half-lives of over 5 hours in
commonly used cell culture media without prior sample processing. Throughput
rates of several thousand samples per hour may be achieved with high
reproducibility under standard laboratory conditions.
Features:
• Greater Light Output: Results in greater assay sensitivity than other
leading extended-lifetime firefly luciferase assay reagents.
• Improved Assay Precision: Less sensitive to mixing conditions for
improved assay reproducibility in multiwell plates. This is particularly useful
in 384-well plates.
• Convenient: Simply mix buffer with lyophilized substrate and use; there is
no need for thawing or measuring before use.
• Quick: No need to remove culture medium or wash cells prior to adding
assay reagent. Cells may be grown and assayed directly within the same
multiwell plate.
• Easy to Use: Simply add reagent, which contains a cell lysis component,
wait 5 minutes and measure luminescence.
• Robust: Compatible with many tissue culture media, including those
containing up to 10% serum.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store Steady-Glo ® Luciferase Assay Substrate at –20°C.
Store Steady-Glo ® Luciferase Assay Buffer below 25°C.
Protocol
Steady-Glo ® Luciferase Assay System Technical Manual
Part#
TM051
Bright-Glo Luciferase Assay System
Product Size Cat.# Price ($)
Bright-Glo Luciferase Assay System 10 ml E2610 239
100 ml E2620 1879
10 × 100 ml E2650 Pls. Enq.
For Laboratory Use.
Description: High-throughput quantitation of firefly (Photinus pyralis)
luciferase expression in mammalian cells requires highly sensitive reagents
that can adapt to continuous-process robotic systems. Bright-Glo Luciferase
Assay System is designed specifically to meet the needs of continuous-process
systems by providing robust, homogeneous assay chemistry that achieves high
assay sensitivity and approximately 30-minute signal half-life without prior
sample processing. These attributes also benefit scientists who are using fewer
samples but still require high sensitivity and ease of use.
Features:
• Quick: No need to remove culture medium or wash cells prior to adding
assay reagent. Cells can be grown and assayed directly within the same
multiwell plate.
• Sensitive: Up to tenfold more light intensity than other homogeneous
luciferase assay reagents results in increased sensitivity.
• Improved Assay Precision: Less sensitive to mixing conditions, sample
evaporation and pipetting errors, resulting in improved assay reproducibility.
• Convenient: Simply mix buffer with lyophilized substrate and add to cells
in culture medium; no need for thawing or measuring before use.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store Bright-Glo Luciferase Assay Substrate at
–20°C. Store Bright-Glo Luciferase Assay Buffer below 25°C.
Protocol
Bright-Glo Luciferase Assay System Technical Manual
Glo Lysis Buffer, 1X
Part#
TM052
Product Size Cat.# Price ($)
Glo Lysis Buffer, 1X 100 ml E2661 136
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Glo Lysis Buffer (GLB), 1X, is a proprietary formulation developed
to promote rapid lysis (within 5 minutes) of cultured mammalian cells without
scraping or performing freeze-thaw cycles. It is fully compatible with Bright-
Glo, Steady-Glo ® , ONE-Glo and Renilla-Glo Luciferase Assay Reagents
and the Luciferase Assay Reagent for analysis of firefly luciferase expression.
The half-life of these reagents remains the same with or without use of GLB,
>5 hours for Steady-Glo ® Reagent and >24 minutes for Bright-Glo Reagent.
Features:
• Convenient: No need for cell scraping or freeze-thaw cycles.
• Fast: Cell lysis within 5 minutes.
• Versatile: Use with Bright-Glo, Steady-Glo ® , ONE-Glo and Renilla-
Glo Luciferase Assay Reagents to provide nonhomogeneous assay
formats or with other reporter applications.
• Robust: Firefly luciferase enzyme in Glo Lysis Buffer is stable at room
temperature for at least 48 hours.
Storage Conditions: Store Glo Lysis Buffer at 4°C. For long-term storage,
Glo Lysis Buffer can be frozen at –20°C or –70°C.
Protocol
Glo Lysis Buffer Protocol
Part#
9PIE266
Section
Contents
Table of
Contents
302
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