2013 Promega catalogue

Cell Signaling
pCI-neo Mammalian Expression Vector
Product Size Cat.# Price ($)
pCI-neo Mammalian Expression Vector 20 μg E1841 455
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The pCI-neo Mammalian Expression Vector carries the human
cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote
constitutive expression of cloned DNA inserts in mammalian cells. This vector
also contains the neomycin phosphotransferase gene, a selectable marker
for mammalian cells. The pCI-neo Vector can be used for transient or stable
expression by selecting transfected cells with the antibiotic G-418.
Features:
• Strong, Constitutive Expression: The human cytomegalovirus (CMV)
immediate-early enhancer/promoter region produces strong, constitutive
expression. A β-globin/IgG chimeric intron located downstream from the
enhancer/promoter region can further increase expression. The vector is
maintained as an episome in cells expressing the SV40 large T antigen,
leading to even higher levels of expression.
• Transient or Stable Expression: The neomycin phosphotransferase
gene allows selection of stable transfected cells.
• Increased Steady-State mRNA Levels: The late SV40 polyadenylation
signal increases the steady-state level of RNA approximately fivefold more
than the early SV40 polyadenylation signal.
• Convenient: Multiple cloning sites exist for easy insertion of cDNA.
• Versatile: Synthesize transcripts in vitro using the T7 RNA polymerase
promoter or generate single-stranded DNA in E. coli using the f1 origin of
replication.
Storage Conditions: Store at –20°C.
Protocol
pCI-neo Mammalian Expression Vector Technical Bulletin
BamHI
Amp r
Synthetic
poly(A)
BglII
ori
CMV I.E.
Enhancer/Promoter
pCI-neo
Vector
(5472bp)
Intron
SV40 Enhancer/
Early Promoter
neo
f1 ori
SgfI
SV40 Late
poly(A)
I-PpoI
T7
NheI
XhoI
EcoRI
MluI
XbaI
SalI
AccI
SmaI
NotI
T3
1085
1091
1096
1102
1114
1120
1121
1127
1131
Part#
TB215
0914VA01_5A
Cell-Free Expression Vectors
In Vitro Translation Specialty Vectors
Product Size Cat.# Price ($)
pF3A WG (BYDV) Flexi ® Vector 20 μg L5671 465
pF3K WG (BYDV) Flexi ® Vector 20 μg L5681 465
pF25A ICE T7 Flexi ® Vector 20 μg L1061 478
pF25K ICE T7 Flexi ® Vector 20 μg L1081 478
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: These Flexi ® vectors are designed with special sequences for
maximal cell-free protein expression in a specific system. The pF3A/K WG
vectors were designed for use with Wheat Germ extracts and contain
sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus,
upstream and downstream of the protein coding region of interest. The BYDV
elements interact with each other, form a closed loop and act synergistically
to stimulate translation in wheat germ extracts, bypassing mRNA cap and
polyadenylation dependencies. The pF25A/K ICE Vectors were designed for use
with Insect Cell Extracts and contain untranslated region (UTR) sequences at
the 5´ and 3´ ends of the gene coding region to enhance translation efficiency.
Note: Flexi ® Vectors contain the lethal barnase gene to reduce background
colonies without inserts during the subcloning procedure. Using the Flexi ®
Vector Cloning System replaces the barnase gene with your insert. These
vectors, as purchased, cannot be cultured in normal laboratory strains of
E. coli without an insert.
Features:
• Versatility: You can choose between a variety of initial applications (e.g.,
bacterial protein, mammalian, or cell-free protein expression) and then
transfer to others as required.
• Time Savings: Efficient transfer allows for direct use of recombinant
clones, minimizing time wasted screening background colonies.
• Enhanced Productivity: Adaptable to high-throughput formats for large
screening projects.
• Easy Access: No licensing fees or complicated transfer restrictions.
Storage Conditions: Store vectors at –20°C.
pTnT Vector
Product Size Cat.# Price ($)
pTnT Vector 20 μg L5610 341
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The pTnT Vector is designed for the convenient in vitro
expression of cloned genes. Both SP6 and T7 polymerase promoters lie in
tandem adjacent to the multiple cloning site. This permits gene expression
from either an SP6- or T7-based coupled in vitro transcription/translation
system. The presence of RNA phage promoters also allows for the highly
efficient synthesis of RNA in vitro. The pTnT Vector also contains a 5´
β-globin leader sequence and synthetic poly(A) 30 tail, both of which have
been shown to enhance expression of certain genes.
Features:
• Flexible: The vector contains tandem SP6 and T7 phage promoters
allowing use in the appropriate in vitro translation or transcription system.
• Convenient: Multiple cloning site provides a selection of restriction sites
for cloning.
Storage Conditions: Store at –20°C.
Protocol
pTnT Vector Technical Bulletin
Part#
TB304
21
Vectors
Section
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