2013 Promega catalogue

Cell Signaling
ReliaPrep Blood gDNA Miniprep System
Product Size Cat.# Price ($)
ReliaPrep Blood gDNA Miniprep System 100 preps A5081 495
For Laboratory Use.
250 preps A5082 1090
Description: The ReliaPrep Blood gDNA Miniprep System provides a
complete, ready-to-use method for purification of gDNA from up to 200μl of
blood or body fluid, consistently isolating pure, intact gDNA without the use of
alcohol washes or precipitations. Genomic DNA can be prepared from fresh
or frozen blood in less than 40 minutes with expected DNA yields of 4–10μg,
depending on the white blood cell count of the blood sample.
Features:
• Easy to Use: Reagents are supplied “ready to go”; no additions required.
• Save Time: Eluted DNA obtained in 30 minutes or less.
• No Ethanol: Eliminates alcohol inhibition and carryover.
• Pure gDNA: Improved A 260 /A 230 ratios vs. the leading competitor.
• Peace of Mind: Consistent results from run to run and between users
even with hemolyzed samples.
• Concentrated DNA: Good recovery and purity in as little as 50μl elution.
Storage Conditions: Store at 15–30°C.
Protocol
ReliaPrep Blood gDNA Miniprep System Technical Manual
ReliaPrep gDNA Tissue Miniprep System
Part#
TM330
Product Size Cat.# Price ($)
ReliaPrep gDNA Tissue Miniprep System 100 preps A2051 495
250 preps A2052 1090
For Laboratory Use.
Description: The ReliaPrep gDNA Tissue Miniprep System provides a
complete, ready-to-use method for purification of gDNA from up to 25mg of
tissue, a buccal (cheek) swab, or a 1cm mouse tail snip, obtaining intact gDNA
without the use of ethanol washes or precipitations.
Features:
• Easy to Use: Reagents are supplied “ready-to-use”—no additions
required.
• Save Time: Eluted DNA obtained in 30 minutes or less (hands-on time).
• No Ethanol: Eliminates alcohol inhibition and carryover.
• Pure gDNA: Improved A 260 /A 230 ratios vs. the leading competitor.
• Peace of Mind: Consistent results from run to run and between users.
• Concentrated DNA: Good recovery and purity in as little as 50μl elution.
Storage Conditions: Store at 15–30°C.
Protocol
ReliaPrep gDNA Tissue Miniprep System Technical Manual
Part#
TM345
ReliaPrep 96 gDNA Miniprep HT System
Product Size Cat.# Price ($)
ReliaPrep 96 gDNA Miniprep HT System 1 × 96 preps A2670 350
4 × 96 preps A2671 1260
Available Separately Size Conc. Cat.# Price ($)
Heat Block Adapter 1 each A2661 582
RNase A Solution 5 ml 4 mg/ml A7974 Pls. Enq.
25mM Tris-HCl (pH 8.0) 60 ml A2641 30
10mM EDTA (pH 8.0) 10 ml A2631 30
20X TE Buffer (pH 7.5) 25 ml A2651 30
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The ReliaPrep 96 gDNA Miniprep HT System provides a simple
and reliable method for the rapid isolation of gDNA in a multiwell format. gDNA
may be purified from blood and Oragene ® •Discover sample collection devices.
The purified gDNA can be used directly in PCR assays, microarrays and nextgeneration
sequencing applications. The use of paramagnetic particles for DNA
capture eliminates the need for centrifugation or vacuum manifolds, making
the system suitable for full automation. In addition, the system does not require
an organic solvent, making it safe and convenient. DNA yields of up to 12µg
are expected from input blood volumes of 350µl, depending on the WBC count
of the sample. Saliva samples can have variable amounts of gDNA, and up
to 18µg or more of DNA may be recovered from a 700µl Oragene ® collection
device sample.
Features:
• Improve Productivity: Walkaway automation of genomic DNA extraction.
• Eliminate Sample Rework: Robust, precipitation-free protocol, no
chance of “lost pellets”.
• Simplify Workflow: High yields of pure DNA from pristine and challenged
or hemolysed samples.
Protocol
ReliaPrep 96 gDNA Miniprep HT System Technical Manual
ReliaPrep 96 gDNA Miniprep HT System Protocol for the Beckman
Coulter Biomek ® FX P
ReliaPrep FFPE gDNA Miniprep System
Part#
TM368
EP045
Product Size Cat.# Price ($)
ReliaPrep FFPE gDNA Miniprep System 10 reactions A2351 150
100 reactions A2352 830
Available Separately Size Cat.# Price ($)
ClickFit Microtube, 1.5ml 1,000 /pack V4741 Pls. Enq.
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The ReliaPrep FFPE gDNA Miniprep System provides a
complete, all-inclusive method for purifying quality genomic DNA from formalinfixed
paraffin-embedded tissue without using hazardous solvents or overnight
digestion. Genomic DNA can be isolated from FFPE tissue in approximately two
and one-half hours with minimal hands-on time.
Features:
• Isolate Quality, Intact gDNA: Optimized lysis and binding conditions
reverse modifications introduced by the fixation process, resulting in intact,
amplifiable gDNA.
• Safely Deparaffinize Your Sample: Deparaffinization step occurs
without harsh organic solvents.
• Save Time: Purify gDNA from FFPE tissue in less than two and one-half
hours with minimal hands-on time. No overnight digestion required.
• Easy to Use: Minimal preparation time; simply add ethanol and go!
Storage Conditions: Store at room temperature.
Protocol
ReliaPrep FFPE gDNA Miniprep System Technical Manual
Part#
TM352
6
DNA and RNA Purification
Section
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