2013 Promega catalogue

Life
Science
Catalog
2013
Worldwide Contact List
Section
Contents
Cell Signaling
HaloTag ® Fusion (N-Terminal) Mammalian
Expression Vectors
Product Size Cat.# Price ($)
pHTN HaloTag ® CMV-neo Vector 20 μg G7721 363
pFN28A HaloTag ® CMV-neo Flexi ® Vector 20 μg G8441 405
pFN28K HaloTag ® CMV-neo Flexi ® Vector 20 μg G8451 405
pFN21A HaloTag ® CMV Flexi ® Vector 20 μg G2821 405
pFN21K HaloTag ® CMV Flexi ® Vector 20 μg G2831 405
pFN22A HaloTag ® CMVd1 Flexi ® Vector 20 μg G2841 405
pFN22K HaloTag ® CMVd1 Flexi ® Vector 20 μg G2851 405
pFN23A HaloTag ® CMVd2 Flexi ® Vector 20 μg G2861 405
pFN23K HaloTag ® CMVd2 Flexi ® Vector 20 μg G2871 405
pFN24A HaloTag ® CMVd3 Flexi ® Vector 20 μg G2881 405
pFN24K HaloTag ® CMVd3 Flexi ® Vector 20 μg G2981 405
For Research Use Only. Not for Use in Diagnostic Procedures.
For additional information see page 274.
Relative Mammalian Protein Expression Levels
for HaloTag ® Flexi ® Vectors.
Vector Name Cat.# Expression Level*
pFC14A HaloTag ® CMV
Flexi ® Vector
pFC14K HaloTag ® CMV
Flexi ® Vector
pFC15A HaloTag ® CMVd1
Flexi ® Vector
pFC15K HaloTag ® CMVd1
Flexi ® Vector
pFC16A HaloTag ® CMVd2
Flexi ® Vector
pFC16K HaloTag ® CMVd2
Flexi ® Vector
pFC17A HaloTag ® CMVd3
Flexi ® Vector
pFC17K HaloTag ® CMVd3
Flexi ® Vector
pFN21A HaloTag ® CMV
Flexi ® Vector
pFN21K HaloTag ® CMV
Flexi ® Vector
pFN22A HaloTag ® CMVd1
Flexi ® Vector
pFN22K HaloTag ® CMVd1
Flexi ® Vector
pFN23A HaloTag ® CMVd2
Flexi ® Vector
pFN23K HaloTag ® CMVd2
Flexi ® Vector
pFN24A HaloTag ® CMVd3
Flexi ® Vector
pFN24K HaloTag ® CMVd3
Flexi ® Vector
pFC27A HaloTag ® CMV-neo
Flexi ® Vector
pFC27K HaloTag ® CMV-neo
Flexi ® Vector
pFN28A HaloTag ® CMV-neo
Flexi ® Vector
pFN27K HaloTag ® CMV-neo
Flexi ® Vector
G9651
G9661
G1611
G1601
G1591
G1571
G1551
G1321
G2821
G2831
G2841
G2851
G2861
G2871
G2881
G2981
High
High
Medium
Medium
Low
Low
Ultra-Low
Ultra-Low
High
High
Medium
Medium
Low
Low
Ultra-Low
Ultra-Low
G8421 High 1
G8431 High 2
G8441 High 1
G8451 High 2 9161LB
ELISAs and Antibodies
BDNF E max
®
ImmunoAssay Systems
Product Size Cat.# Price ($)
BDNF E
®
max ImmunoAssay System 2 × 96 wells G7610 668
5 × 96 wells G7611 1270
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The BDNF E max
®
ImmunoAssay Systems provide optimized
reagents and a protocol for the sensitive and specific detection of brainderived
neurotrophic factor (BDNF). After an overnight coating of a 96-well
plate, the specific protein is detected using an antibody sandwich format. The
systems use a horseradish peroxidase-conjugated secondary antibody and a
single-component TMB substrate for the final chromogenic detection of bound
neurotrophic factor. Using this system, BDNF in tissue culture supernatants,
tissue homogenates, plasma and urine can be quantitated in the range of
7.8–500pg/ml. Binding and recovery from mouse brain homogenates has not
been fully characterized.
Features:
• High Value: Optimized reagents and protocol provided.
• Specificity: Specific detection of BDNF; less than 3% cross-reactivity with
other related neurotrophic and growth factors.
• Sensitivity: Detect picogram levels of factor per milliliter of sample.
• Flexibility: Available kits provide sufficient reagents for two or five 96-well
plates; you can configure your plates as desired.
Plates are not included.
Storage Conditions: Store the entire system in its original package protected
from light at –20°C.
Protocol
®
BDNF E max ImmunoAssay System Technical Bulletin
GDNF E max
®
ImmunoAssay Systems
Part#
TB257
Product Size Cat.# Price ($)
GDNF E
®
max ImmunoAssay System 2 × 96 wells G7620 668
5 × 96 wells G7621 1270
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The GDNF E max
®
ImmunoAssay Systems provide optimized
reagents and a protocol for the sensitive and specific detection of glial cellline-derived
neurotrophic factor (GDNF). After an overnight coating of a 96-well
plate, the specific protein is detected using an antibody sandwich format. The
systems use horseradish peroxidase-conjugated secondary antibody and a
single-component TMB substrate for the final chromogenic detection of bound
neurotrophic factor. Using this system, GDNF in tissue culture supernatants or
tissue homogenates can be quantitated in the range of 15.6–1,000pg/ml.
Features:
• High Value: Optimized reagents and protocol provided.
• Specificity: Specific detection of GDNF; less than 3% cross-reactivity with
other related neurotrophic and growth factors.
• Sensitivity: Detect picogram levels of factor per milliliter of sample.
• Flexibility: Available kits provide sufficient reagents for two or five 96-well
plates; you can configure your plates as desired.
Plates are not included.
Storage Conditions: Store the entire system in its original package protected
from light at –20°C. Once thawed, store the system (except the GDNF Standard)
at 4°C.
Protocol
®
GDNF E max Immunoassay System Technical Bulletin
Part#
TB221
Table of
Contents
196
For complete and up-to-date product information visit: www.promega.com/catalog