2013 Promega catalogue

Cell Signaling
Wizard ® SV 96 and SV 9600 Plasmid DNA
Purification Systems
Product Size Cat.# Price ($)
Wizard ® SV 96 Plasmid DNA Purification
System
Wizard ® SV 9600 Plasmid DNA Purification
System
1 × 96 preps A2250 465
5 × 96 preps A2255 1843
100 × 96 preps A2258 Pls. Enq.
Available Separately Size Cat.# Price ($)
Column Wash Solution (CWA) 185 ml A1311 156
Column Wash Solution (CWA) 370 ml A1318 273
Wizard ® SV 96 Neutralization Solution 500 ml A1481 156
950 ml A1488 217
Wizard ® SV 96 Cell Resuspension Solution 500 ml A7113 151
800 ml A7118 394
Wizard ® SV 96 Cell Lysis Solution 500 ml A7123 151
800 ml A7128 217
Nuclease-Free Water 150 ml P1195 125
Alkaline Protease Solution 3 ml A1441 66
Wizard ® SV 96 Binding Plates 10 pack A2271 1450
100 pack A2278 Pls. Enq.
Wizard ® SV 96 Lysate Clearing Plates 10 pack A2241 1072
100 pack A2248 Pls. Enq.
A1311, A7113, A7123 For Laboratory Use. A2248, A2241, A2278, A2271, A1441, P1195,
A7128, A7118, A1488, A2258, A1481, A2255, A1318, A2250 For Research Use Only. Not for
Use in Diagnostic Procedures.
Description: The Wizard ® SV 96 and SV 9600 Plasmid DNA Purification
Systems provide a simple and reliable method for the rapid isolation of plasmid
DNA using a silica-membrane, 96-well, high-throughput format. A single plate
can be processed in 60 minutes or less. The purified plasmid can be used
directly for automated fluorescent DNA sequencing as well as for other
standard molecular biology techniques, including restriction enzyme digestion.
The Wizard ® SV 96 and SV 9600 Systems are designed for use either in a
manual format or with Beckman Coulter or PerkinElmer automated
instruments.
Features:
• Performance by Design: Vac-Man ® 96 Vacuum Manifold eliminates
waste handling and allows simultaneous lysate clearing and DNA binding.
Novel plate design prevents cross-contamination during sample
processing.
• Flexibility: Designed for use in both manual and automated formats.
• Confidence in Results: Purified DNA meets a target of >98% accuracy
over 600 bases using pGEM ® -3Zf(+) Vector DNA in BigDye ® terminator
sequencing.
• Automation: Validated automated methods available at:
www.promega.com/automethods/
• Your Choice of Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at 22–25°C.
Electropherogram of plasmid DNA sequence following isolation by
the Wizard ® SV 96 System and cycle sequencing using BigDye ®
terminator reactions. Results demonstrate greater than 600 consecutive
bases analyzed with greater than 98% accuracy of base identity.
2687TA
6
DNA and RNA Purification
Protocol
Wizard ® SV 96 Plasmid DNA Purification System Technical Bulletin
Wizard ® SV 9600 Plasmid DNA Purification System Technical
Bulletin
Part#
TB272
TB292
Section
Contents
For complete and up-to-date product information visit: www.promega.com/catalog
139
Table of
Contents