2013 Promega catalogue

Life
Science
Catalog
2013
Worldwide Contact List
Cell Signaling
GST Protein Purification
MagneGST Protein Purification System
Product Size Cat.# Price ($)
MagneGST Protein Purification System 40 reactions V8600 250
200 reactions V8603 860
Available Separately Size Cat.# Price ($)
MagneGST Glutathione Particles 4 ml V8611 191
For Research Use Only. Not for Use in Diagnostic Procedures.
20 ml V8612 696
Description: The MagneGST Protein Purification System provides a simple,
rapid and reliable method for the purification of glutathione-S-transferase (GST)
fusion proteins. Immobilized glutathione paramagnetic particles (MagneGST
Particles) are used to isolate GST-tagged protein directly from a crude or
cleared lysate using either a manual or automated procedure and requires use
of a magnetic stand. GST-tagged proteins can be purified on a small scale from
1ml of culture or on a large scale using more than 50ml of culture. Samples
also can be processed using a robotic platform.
Features:
• Simple: One-step purification of multiple samples with easy handling.
• Quick: After cell lysis, no requirement for high-speed centrifugation to
clear lysate.
• Scalable: Scalable protocol using 1–50ml of cell culture.
• Efficient: Achieve high yields with little or no nonspecific background.
• Complete: System contains all necessary components except magnetic
stand.
Storage Conditions: The complete system consists of two individual parts,
each with a different storage condition. Store individual boxes at specified
temperatures of 4°C and –70°C.
Protocol
MagneGST Protein Purification System Technical Manual
MagneGST Pull-Down System
Part#
TM240
Product Size Cat.# Price ($)
MagneGST Pull-Down System 80 reactions V8870 913
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The MagneGST Pull-Down System is designed for detection of
protein interactions between GST-fusion proteins expressed in bacterial lysates
and prey proteins expressed in the TnT ® Systems. Prey protein synthesized
in the TnT ® Quick Coupled Transcription/Translation Reaction is captured
using bait protein (GST-fusion protein) immobilized on MagneGST Particles.
Nonspecifically bound proteins are then washed away, and the prey protein is
analyzed. Prey proteins can be detected by incorporating radioactively labeled
methionine in the TnT ® Quick reaction, followed by SDS-PAGE and autoradiography
or by incorporating the supplied non-radioactive methionine in the TnT ®
reaction and detecting by Western blotting with protein-specific antibodies.
Storage Conditions: Store the TnT ® T7 Quick Master Mix and Methionine at
–70°C. Store the RQ1 RNase-Free DNase at –20°C. Store the Nuclease-Free
Water, MagneGST Glutathione Particles, MagneGST Binding/Wash Buffer
and Cell Lysis Reagent at 4°C.
Polyhistidine His-Tag Protein
Purification
Maxwell ® 16 Polyhistidine Protein
Purification Kit
Product Size Cat.# Price ($)
Maxwell ® 16 Polyhistidine Protein Purification Kit 48 preps AS1060 Pls. Enq.
Available Separately Size Cat.# Price ($)
Plungers 50 /pk AS5201 28
Elution Tubes 50 /pk AS5101 10
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Maxwell ® 16 Polyhistidine Protein Purification Kit is used
with the Maxwell ® 16 Instrument to provide an easy method for the efficient,
automated purification of polyhistidine-tagged protein from bacterial cultures
and other sample types including mammalian and insect cells. With minor
modifications, the reagents can also be used for purification of HQ-tagged
proteins from bacterial cultures.
The Maxwell ® Instrument is supplied with a preprogrammed purification
procedure and reagent cartridges specifically designed to maximize
simplicity and convenience. The instrument can process up to 16 samples in
approximately 40 minutes. The purified protein is compatible with downstream
applications such as gel electrophoresis and Western blot analysis.
Features:
• Choose Your Sample Type: Flexibility to purify from multiple starting
cultures including bacterial culture, mammalian cells, insect cells and
culture medium.
• Have Confidence in Your Results: Achieve consistent purification across
all samples.
• Save Hands-On Time: Prefilled cartridges eliminate reagent preparation,
multiple pipetting steps, centrifugation and additional sample manipulation.
Storage Conditions: Store at 4°C.
Protocol
Maxwell ® 16 Polyhistidine Protein Purification Kit Technical Manual
Part#
TM285
Protocol
MagneGST Pull-Down System Technical Manual
Part#
TM249
Section
Contents
Table of
Contents
288
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