2013 Promega catalogue

Life
Science
Catalog
2013
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Cell Signaling
FluoroTect Green Lys in vitro Translation
Labeling System
Product Size Cat.# Price ($)
FluoroTect Green Lys in vitro Translation
Labeling System
For Research Use Only. Not for Use in Diagnostic Procedures.
40 reactions L5001 646
Description: The FluoroTect Green Lys in vitro Translation Labeling System
allows for the fluorescent labeling and detection of proteins synthesized in vitro.
The system is based on a lysine-charged tRNA that is labeled at the ε position
of the lysine with the fluorophore BODIPY ® -FL. Fluorescent lysine residues will
be incorporated into synthesized proteins during in vitro translation reactions,
eliminating the need for radioactivity.
Detection of the labeled proteins is accomplished in 2–5 minutes directly
“in-gel” by use of a laser-based fluorescent gel scanner. This eliminates any
requirements for protein gel manipulation such as fixing/drying or any safety,
regulatory and waste disposal issues associated with the use of radioactively
labeled amino acids use. The convenience of “in-gel” detection also avoids
the time-consuming electroblotting and detection steps of conventional nonisotopic
systems.
Features:
• Fast: Data can be obtained in minutes, eliminating overnight exposures
associated with radioactive-based systems or time-consuming steps
utilized by traditional non-isotopic methodologies.
• Convenient: Results based on “in-gel” detection. No requirement to
transfer, fix, or dry gels.
• Non-Radioactive: No safety, regulatory or waste disposal issues
associated with radioactivity.
• Flexible: The modified charged tRNA can be used with a variety of
Promega translation systems including: Rabbit Reticulocyte Lysate,
TnT ® Coupled Transcription/Translation System, Wheat Germ Extract and
E. coli S30 Extract.
Storage Conditions: Store at –70°C.
Protocol
FluoroTect Green Lys in vitro Translation Labeling System
Technical Bulletin
Part#
TB285
Transcend Non-Radioactive Translation
Detection Systems
Product Size Cat.# Price ($)
Transcend Colorimetric Translation Detection 30 reactions L5070 377
System
Transcend Chemiluminescent Translation 30 reactions L5080 566
Detection System
Available Separately Size Cat.# Price ($)
Transcend tRNA 30 μl L5061 307
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Transcend Non-Radioactive Translation Detection
Systems allow non-radioactive detection of proteins synthesized in vitro.
Using these systems, biotinylated lysine residues are incorporated into
nascent proteins during translation, eliminating the need for labeling with
[ 35 S]methionine or other radioactive amino acids. This biotinylated lysine is
added to the translation reaction as a precharged ε-labeled biotinylated lysinetRNA
complex (Transcend tRNA) rather than a free amino acid. After SDS-
PAGE and electroblotting, the biotinylated proteins can be visualized by binding
either Streptavidin-Alkaline Phosphatase (Streptavidin-AP) or Streptavidin-
Horseradish Peroxidase (Streptavidin-HRP), followed either by colorimetric or
chemiluminescent detection. Typically, these methods can detect 0.5–5ng of
protein within 3–4 hours after gel electrophoresis. This sensitivity is equivalent
to that achieved with [ 35 S]methionine incorporation and autoradiographic
detection 6–12 hours after gel electrophoresis.
Features:
• Sensitive: The biotin tag allows detection of 0.5–5ng of translated protein.
• Safe: No radioisotope handling, storage or disposal is required.
• Fast: Labeled proteins can be detected 3–4 hours after gel electrophoresis.
• Flexible: Results can be visualized by using either colorimetric or
chemiluminescent detection.
Storage Conditions: Store Transcend tRNA at –70°C. Do not subject
the Transcend tRNA to more than five freeze-thaw cycles. Store all other
components at 4°C.
Protocol
Transcend Non-Radioactive Translation Detection System
Technical Bulletin
Part#
TB182
Section
Contents
Table of
Contents
276
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