2013 Promega catalogue
2013 Promega catalogue
2013 Promega catalogue
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Cell Signaling<br />
Conventional DNA Markers<br />
Product Size Conc. Cat.# Price ($)<br />
Lambda DNA/HindIII Markers 100 μg 0.5 µg/µl G1711 81<br />
Lambda DNA/EcoRI Markers 100 μg 0.5 µg/µl G1721 81<br />
Lambda DNA/EcoRI + HindIII Markers 100 μg 0.5 µg/µl G1731 81<br />
ΦX174 DNA/HaeIII Markers 50 μg 1 µg/µl G1761 221<br />
ΦX174 DNA/HinfI Markers 50 μg 1 µg/µl G1751 221<br />
pGEM ® DNA Markers 50 μg 1 µg/µl G1741 171<br />
For Laboratory Use.<br />
Description: The Conventional DNA Digest Markers are created by digesting<br />
either λ DNA, ΦX174 replicative form DNA, or plasmids to completion with<br />
one or more restriction enzymes. The enzymes are heat-inactivated, and the<br />
DNA fragments are either phenol-extracted, then ethanol-precipitated or just<br />
ethanol-precipitated. The precipitated fragments are resuspended in storage<br />
buffer. The markers are not intended for quantitative analysis. Each marker is<br />
supplied with a tube of 6X Blue/Orange Loading Dye.<br />
1258TB09_5A<br />
bp<br />
– 23,130<br />
– 9,416<br />
– 6,557<br />
– 4,361<br />
– 2,322<br />
– 2,027<br />
[564,<br />
125]<br />
0.7% agarose<br />
1258TA09_5A<br />
bp<br />
0.7% agarose<br />
– 21,226<br />
– 7,421<br />
5,804<br />
5,643<br />
– 4,878<br />
– 3,530<br />
1258TC09_5A<br />
bp<br />
– 21,226<br />
5,148<br />
4,973<br />
– 4,268<br />
– 3,530<br />
– 1,584<br />
– 1,375<br />
– 947<br />
– 831<br />
0.7% agarose<br />
– 2,027<br />
––––––– 1,904<br />
[564,<br />
125]<br />
λ DNA/HindIII Markers: Eight ethanol-precipitated DNA fragments ranging in<br />
size from 125bp to 23,130bp.<br />
λ DNA/EcoRI Markers: Six ethanol-precipitated DNA fragments ranging in<br />
size from 3,530bp to 21,226bp.<br />
λ DNA/EcoRI + HindIII Markers: Thirteen ethanol-precipitated DNA fragments<br />
ranging in size from 125bp to 21,226bp.<br />
ΦX174 DNA/HaeIII Markers: Eleven phenol-extracted, ethanol-precipitated<br />
DNA fragments ranging in size from 72bp to 1,353bp.<br />
ΦX174 DNA/HinfI Markers: Twenty ethanol-precipitated DNA fragments<br />
ranging in size from 24bp to 726bp.<br />
pGEM ® DNA Markers: Fifteen phenol-extracted, ethanol-precipitated DNA<br />
fragments ranging in size from 36bp to 2,645bp. These unique markers are<br />
generated from separate digests of pGEM ® -3 Vector DNA with HinfI, RsaI and<br />
AvaII later combined to form the markers.<br />
Recommended Loading: Load 1μl/lane.<br />
Storage Conditions: Store at –20°C.<br />
1238TA09_5A<br />
bp<br />
– 1,353<br />
– 1,078<br />
– 872<br />
– 603<br />
– 310<br />
– 281<br />
––––– 271<br />
– 234<br />
– 194<br />
– 118<br />
[72]<br />
8% acrylamide<br />
0265TA05_2A<br />
bp<br />
–<br />
–––––<br />
726<br />
713<br />
– 553<br />
– 500<br />
– 311<br />
– 249<br />
– 200<br />
8% acrylamide<br />
427<br />
417<br />
413<br />
–––––<br />
– 151<br />
140<br />
– 118<br />
– 100<br />
– 82<br />
– 66<br />
48<br />
42<br />
– 24 40<br />
0266TA05_1A<br />
bp<br />
– 2,645<br />
– 1,605<br />
– 1,198<br />
– 676<br />
– 517<br />
– 460<br />
– 396<br />
– 350<br />
– 222<br />
– 179<br />
– 126<br />
– 75<br />
– 65<br />
– 51<br />
[36]<br />
8% acrylamide<br />
8655TA<br />
5<br />
Cloning and DNA Markers<br />
Lambda DNA/<br />
HindIII Markers<br />
Cat.# G1711<br />
Load 1µl/lane.<br />
Lambda DNA/<br />
EcoRI Markers<br />
Cat.# G1721<br />
Load 1µl/lane.<br />
Lambda DNA/EcoRI +<br />
HindIII Markers<br />
Cat.# G1731<br />
Load 1µl/lane.<br />
φX174 DNA/<br />
HaeIII Markers<br />
Cat.# G1761<br />
Load 1µl/lane.<br />
φX174 DNA/<br />
HinfI Markers<br />
Cat.# G1751<br />
Load 1µl/lane.<br />
pGEM ® DNA<br />
Markers<br />
Cat.# G1741<br />
Load 1µl/lane.<br />
Section<br />
Contents<br />
For complete and up-to-date product information visit: www.promega.com/catalog<br />
91<br />
Table of<br />
Contents
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