2013 Promega catalogue

Cell Signaling
Pfu DNA Polymerase
dNTP Mix
Product Size Conc. Cat.# Price ($)
Pfu DNA Polymerase 100 u 2–3 u/µl M7741 193
500 u 2–3 u/µl M7745 725
For Research Use Only. Not for Use in Diagnostic Procedures. Product may not be available in
all countries. Please contact your local representative for more information.
Description: Pfu DNA Polymerase is a thermostable enzyme of approximately
90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C,
catalyzing the polymerization of nucleotides into duplex DNA in the 5´→3´
direction in the presence of magnesium. Pfu DNA Polymerase also possesses
3´→5´ exonuclease (proofreading) activity. Base misinsertions that may occur
during polymerization are rapidly excised by the proofreading activity of the
polymerase. Consequently, Pfu DNA Polymerase is recommended for use in
PCR and primer extension reactions that require high-fidelity synthesis. Pfu
DNA Polymerase-generated PCR fragments are blunt-ended.
Pfu DNA Polymerase 10X Reaction Buffer with MgSO 4 : 200mM Tris-HCl
(pH 8.8 at 25°C), 100mM KCl, 100mM (NH 4 ) 2 SO 4 , 20mM MgSO 4 , 1.0% Triton ®
X-100 and 1mg/ml nuclease-free BSA.
Features:
• High Fidelity: Pfu DNA Polymerase exhibits the lowest error rate of any
thermostable DNA polymerase.
• Complete: Provided with 10X buffer containing 20mM MgSO 4 .
• Performance Guarantee: Promega PCR systems, enzymes and reagents
are proven in PCR to ensure reliable, high-performance results. If you are
not completely satisfied with any Promega PCR product, we will send a
replacement or refund your account.
Storage Conditions: Store at –20°C.
Protocol
Pfu DNA Polymerase Protocol
PCR Nucleotide Mix
Part#
9PIM774
Product Size Conc. Cat.# Price ($)
PCR Nucleotide Mix 200 μl 10 mM C1141 111
1,000 μl 10 mM C1145 412
For Laboratory Use.
Description: High-quality deoxynucleotide triphosphates (dNTPs) are critical for
PCR success. The PCR Nucleotide Mix is a premixed solution containing sodium
salts of dATP, dCTP, dGTP and dTTP, each at a concentration of 10mM in water
at pH 7.5; the total concentration of nucleotides is 40mM. This solution is ready
to use and is optimized for standard PCR and specialty approaches including
hot-start and reverse transcription PCR (RT-PCR). One microliter (1μl) is sufficient
for amplification in a typical 50μl reaction volume.
Features:
• Optimized and Pretested in PCR: Equimolar amounts of each dNTP
ensure optimal PCR.
• Convenient: Add 1µl for 50μl PCR.
• Easy to Use: Reduced pipetting steps contribute to ease-of-use and
reduce the risk of contamination.
• Performance Guaranteed: Promega PCR systems, enzymes and
reagents are proven in PCR to ensure reliable, high-performance results.
If you are not completely satisfied with any Promega PCR product, we will
send a replacement or refund your account.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –20°C. Avoid exposure to frequent temperature
changes.
Protocol
PCR Nucleotide Mix Protocol
Part#
9PIC114
For complete and up-to-date product information visit: www.promega.com/catalog
Product Size Conc. Cat.# Price ($)
dNTP Mix 200 μl 10 mM U1511 41
1,000 μl 10 mM U1515 98
For Laboratory Use.
Description: dNTP Mix is a premixed solution containing sodium salts of dATP,
dCTP, dGTP and dTTP, each at 10mM in water at pH 7.5; the total concentration
of nucleotides is 40mM. One microliter of the dNTP Mix in a 50μl reaction will
give a final dNTP concentration of 200μM for each dNTP.
Features:
• High Purity: dNTPs are >98% pure.
• Ease to Use: Reduced pipetting steps contribute to ease-of-use and
reduce the risk of contamination.
Storage Conditions: Store at –20°C. Avoid exposure to frequent temperature
changes.
Deoxynucleotide Triphosphates (dNTPs)
Product Size Conc. Cat.# Price ($)
dATP 25 μmol 100 mM U1205 86
40 μmol 100 mM U1201 110
200 μmol 100 mM U1202 441
dGTP 25 μmol 100 mM U1215 86
40 μmol 100 mM U1211 110
200 μmol 100 mM U1212 441
dCTP 25 μmol 100 mM U1225 86
40 μmol 100 mM U1221 110
200 μmol 100 mM U1222 441
dTTP 25 μmol 100 mM U1235 86
40 μmol 100 mM U1231 110
200 μmol 100 mM U1232 441
Set of dATP, dCTP, dGTP, dTTP 10µmol each 100 mM U1330 110
25 μmol each 100 mM U1420 250
40µmol each 100 mM U1240 389
200 μmol 100 mM U1410 1351
For Laboratory Use.
Description: High-quality deoxynucleotide triphosphates (dNTPs) are critical
for the success of many key procedures such as cDNA synthesis, sequencing
and labeling. Promega dNTPs have greater than 99% triphosphate content and
are provided at a concentration of 100mM in water at pH 7.5.
Features:
• Dependable: PCR-tested deoxynucleotides ensure optimal performance
with all Promega amplification enzymes.
• Consistent: dNTPs are >99% pure, allowing highly consistent results.
• Convenient: Supplied at a convenient concentration (100mM in water) for
ease-of-use in PCR and other applications.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –30°C to –10°C. Avoid exposure to frequent
temperature changes.
PCR Amplifications From Each Size of Individual dNTPs.
Each catalog number supplies each individual dNTP at 100mM. Reactions
are based on 200µM each dNTP in a 50µl reaction.
Cat.# Quantity Volume Reactions
U1330, U1335 10 µmol each 100 µl each 1,000
U1420 25 µmol each 250 µl each 2,500
U1240, U1245 40 µmol each 400 µl each 4,000
U1410 200 µmol each 2 × 1,000 µl each 20,000
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PCR
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