2013 Promega catalogue
2013 Promega catalogue
2013 Promega catalogue
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Life<br />
Science<br />
Catalog<br />
<strong>2013</strong><br />
Worldwide Contact List<br />
Cell Signaling<br />
HaloTag ® Ligand Building Blocks<br />
Product Size Cat.# Price ($)<br />
HaloTag ® Amine (O4) Ligand 5 mg P6741 928<br />
HaloTag ® Amine (O2) Ligand 5 mg P6711 928<br />
HaloTag ® Iodoacetamide (O4) Ligand 5 mg P6771 923<br />
HaloTag ® Iodoacetamide (O2) Ligand 5 mg P1681 923<br />
HaloTag ® Succinimidyl Ester (O4) Ligand 5 mg P6751 923<br />
HaloTag ® Succinimidyl Ester (O2) Ligand 5 mg P1691 923<br />
HaloTag ® Thiol (O4) Ligand 5 mg P6761 923<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
Description: The HaloTag ® Ligand Building Blocks can carry a variety of<br />
functionalities, including fluorescent labels, affinity tags and attachments to<br />
a solid phase. The covalent bond forms rapidly under general physiological<br />
conditions, is highly specific and essentially irreversible. The HaloTag ® Ligand<br />
Building Blocks allow researchers to apply the chloroalkane group that<br />
HaloTag ® protein reacts with to any compound or surface with a compatible<br />
chemical group, creating endless possible applications.<br />
The HaloTag ® Succinimidyl Ester (04) Ligand contains a reactive succinimidyl<br />
ester (SE) group connected to an alkyl chloride separated by three ethylene<br />
glycol repeats (04). The HaloTag ® Succinimidyl Ester (04) Ligand can be<br />
successfully conjugated to any reporter group, protein, or nucleic acid<br />
derivative containing an amine, forming stable amide bond linkages. The<br />
ligand with functional group can then be used with the HaloTag ® protein for<br />
any application of interest.<br />
The HaloTag ® Succinimidyl Ester (O2) Ligand contains a reactive succinimidyl<br />
ester (SE) group connected to an alkylchloride separated by an ethylene glycol<br />
repeat (O2). The HaloTag ® Succinimidyl Ester (O2) Ligand can be successfully<br />
conjugated to any reporter group, protein, or nucleic acid derivative containing<br />
an amine, forming stable amide bond linkages. The ligand with functional group<br />
can then be used with the HaloTag ® protein for any application of interest.<br />
The HaloTag ® Amine (04) Ligand contains a reactive amine group<br />
connected to an alkyl chloride, separated by an ethylene glycol repeat (04).<br />
The HaloTag ® Amine (04) Ligand can be successfully conjugated to any<br />
reporter group, protein, or nucleic acid derivative containing an activated<br />
carboxylic acid, sulfonyl halide or isocyanate. Examples of activated carboxylic<br />
acids are succinimidyl esters, STP esters, acid halides, and TFP esters. The<br />
ligand with functional group can then be used with the HaloTag ® protein for<br />
any application of interest.<br />
The HaloTag ® Amine (O2) Ligand contains a reactive amine group<br />
connected to an alkylchloride, separated by an ethylene glycol repeat (O2).<br />
The HaloTag ® Amine (O2) Ligand can be successfully conjugated to any<br />
reporter group, protein, or nucleic acid derivative containing an activated<br />
carboxylic acid, sulfonyl halide, or isocyanate. Examples of activated carboxylic<br />
acids are succinimidyl esters, STP esters, acid halides, and TFP esters. The<br />
ligand with functional group can then be used with the HaloTag ® protein for<br />
any application of interest.<br />
The HaloTag ® Iodoacetamide (04) Ligand contains a reactive iodoacetamide<br />
group connected an alkyl chloride separated by an ethylene glycol repeat (04).<br />
The HaloTag ® Iodoacetamide (04) Ligand has been designed to rapidly react<br />
with sulfhydryl-containing molecules, whether small organic compounds,<br />
peptides or proteins. The ligand with functional group can then be used with<br />
the HaloTag ® protein for any application of interest.<br />
The HaloTag ® Iodoacetamide (O2) Ligand contains a reactive iodoacetamide<br />
group connected to an alkylchloride separated by an ethylene glycol repeat<br />
(O2). HaloTag ® Iodoacetamide (O2) Ligand has been designed to rapidly react<br />
with sulfhydryl-containing molecules, whether small organic compounds,<br />
peptides or proteins. The ligand with functional group can then be used with<br />
the HaloTag ® protein for any application of interest.<br />
The HaloTag ® Thiol (O4) Ligand contains a reactive sulfhydryl group connected<br />
to an alkyl chloride separated by three ethylene glycol repeats (04). The<br />
HaloTag ® Thiol (04) Ligand can be successfully conjugated to any reporter<br />
group, cross-linking reagent (bound or free), or nucleic acid derivative<br />
containing a number of different alkylating groups, forming stable thioether<br />
bonds. Commonly used reagents that rapidly react with sulfhydryls include<br />
iodo- or bromo-acetyls or benzyls, bromo- or chloro-mustards, maleimides,<br />
aziridines, acryloyl derivatives, and halide or sulfonate containing arenes (those<br />
bearing Electron Withdrawing Groups (EWGs) react most rapidly). The reactive<br />
ligand can be captured with HaloTag ® protein either before or after the thiol<br />
group is functionalized for any application of interest.<br />
Storage Conditions: Store Cat.# P1691 and P6751 at or below –70°C<br />
under inert atmosphere. Store Cat.# P6711 and P6741 at or below –20°C in<br />
an air-tight container in the absence of light. Store Cat.# P1681, P6771 and<br />
P6761 at or below –20°C under inert atmosphere in the absence of light. See<br />
<strong>Promega</strong> Product Information for additional details on individual products.<br />
Section<br />
Contents<br />
Table of<br />
Contents<br />
272<br />
For complete and up-to-date product information visit: www.promega.com/catalog
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