2013 Promega catalogue

Cell Signaling
DNA Purification Technologies
ReliaPrep Large Volume HT gDNA Isolation
System
Product Size Cat.# Price ($)
ReliaPrep Large Volume HT
96 × 10ml preps A1751 Pls. Enq.
gDNA Isolation System
Available Separately Size Cat.# Price ($)
ReliaPrep LV 32 HSM Instrument 1 each A1715 Pls. Enq.
A1751 For Laboratory Use. A1715 For Research Use Only. Not for Use in Diagnostic
Procedures. Product may not be available in all countries. Please contact your local
representative for more information.
Description: The ReliaPrep Large Volume HT gDNA Isolation System
isolates genomic DNA (gDNA) from 3–10ml of blood in a scalable format. The
chemistry eliminates tedious centrifugation steps as well as the use of
hazardous chemicals, which are inherent in precipitation-based chemistries.
The system has been automated on robotic liquid-handling workstations,
allowing walkaway purification of genomic DNA from 3–10ml of whole blood,
regardless of sample storage or shipping conditions. For low-throughput
isolation of gDNA from up to 32 samples at one time, the ReliaPrep LV 32
HSM can be used in a manual mode, where the user performs the
pipetting functions. The HSM has an LCD screen that directs the user through
the protocol.
Storage Conditions: Store at 15–30°C.
Protocol
ReliaPrep Large Volume HT gDNA Isolation System Technical
Manual
ReliaPrep LV 32 HSM Instrument Technical Manual
ReliaPrep Blood gDNA Miniprep System
Part#
TM341
TM326
Product Size Cat.# Price ($)
ReliaPrep Blood gDNA Miniprep System 100 preps A5081 495
250 preps A5082 1090
For Laboratory Use.
Description: The ReliaPrep Blood gDNA Miniprep System provides a
complete, ready-to-use method for purification of gDNA from up to 200μl of
blood or body fluid, consistently isolating pure, intact gDNA without the use of
alcohol washes or precipitations. Genomic DNA can be prepared from fresh
or frozen blood with expected DNA yields of 4–10μg, depending on the white
blood cell count of the blood sample.
Features:
• Easy to Use: Reagents are supplied “ready to go”; no additions required.
• Save Time: Eluted DNA obtained in 30 minutes or less.
• No Ethanol: Eliminates alcohol inhibition and carryover.
• Pure gDNA: Improved A 260 /A 230 ratios vs. the leading competitor.
• Peace of Mind: Consistent results from run to run and between users
even with hemolyzed samples.
• Concentrated DNA: Good recovery and purity in as little as 50μl elution.
Storage Conditions: Store at 15–30°C.
ReliaPrep gDNA Tissue Miniprep System
Product Size Cat.# Price ($)
ReliaPrep gDNA Tissue Miniprep System 100 preps A2051 495
250 preps A2052 1090
For Laboratory Use.
Description: The ReliaPrep gDNA Tissue Miniprep System provides a
complete, ready-to-use method for purification of gDNA from up to 25mg of
tissue, a buccal (cheek) swab, or a 1cm mouse tail snip, obtaining intact gDNA
without the use of ethanol washes or precipitations.
Features:
• Easy to Use: Reagents are supplied “ready-to-use”—no additions
required.
• Save Time: Eluted DNA obtained in 30 minutes or less (hands-on time).
• No Ethanol: Eliminates alcohol inhibition and carryover.
• Pure gDNA: Improved A 260 /A 230 ratios vs. the leading competitor.
• Peace of Mind: Consistent results from run to run and between users.
• Concentrated DNA: Good recovery and purity in as little as 50μl elution.
Storage Conditions: Store at 15–30°C.
Protocol
ReliaPrep gDNA Tissue Miniprep System Technical Manual
ReliaPrep FFPE gDNA Miniprep System
Part#
TM345
Product Size Cat.# Price ($)
ReliaPrep FFPE gDNA Miniprep System 10 reactions A2351 150
100 reactions A2352 830
Available Separately Size Cat.# Price ($)
ClickFit Microtube, 1.5ml 1,000 /pack V4741 Pls. Enq.
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The ReliaPrep FFPE gDNA Miniprep System provides a
complete, all-inclusive method for purifying quality genomic DNA from formalinfixed
paraffin-embedded tissue without using hazardous solvents or overnight
digestion. Genomic DNA can be isolated from FFPE tissue in approximately two
and one-half hours with minimal hands-on time.
Features:
• Isolate Quality, Intact gDNA: Optimized lysis and binding conditions
reverse modifications introduced by the fixation process, resulting in intact,
amplifiable gDNA.
• Safely Deparaffinize Your Sample: Deparaffinization step occurs
without harsh organic solvents.
• Save Time: Purify gDNA from FFPE tissue in less than two and one-half
hours with minimal hands-on time. No overnight digestion required.
• Easy to Use: Minimal preparation time; simply add ethanol and go!
Storage Conditions: Store at room temperature.
Protocol
ReliaPrep FFPE gDNA Miniprep System Technical Manual
Part#
TM352
8
Epigenetics
Protocol
ReliaPrep Blood gDNA Miniprep System Technical Manual
Part#
TM330
Section
Contents
For complete and up-to-date product information visit: www.promega.com/catalog
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