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23<br />

Switzer<strong>la</strong>nd). To check sex and repro<strong>du</strong>ctive status of an indivi<strong>du</strong>al, we twisted the n<strong>et</strong><br />

several times to restrain its movements. We d<strong>et</strong><strong>et</strong>mined male repro<strong>du</strong>ctive status from the<br />

abdominal or sc rotai position of testicles, and female repro<strong>du</strong>ctive status by palpation and<br />

manual extraction ofmilk (Ilse & Hellgren 2001). We used chemical immobilization (Morin<br />

& Berteaux 2003) only when marking or col<strong>la</strong>ring porcupines.<br />

Marking<br />

We ear-tagged porcupines with a unique combination oftwo color- and sign-co<strong>de</strong>d<br />

p<strong>la</strong>stic tags (15 x45 mm, modified from Sweitzer and Berger (1992), Alltlex Canada, St-<br />

Hyacinthe, Que.) to allow i<strong>de</strong>ntification at a distance. Both tags bore the same sign but colors<br />

were in<strong>de</strong>pen<strong>de</strong>nt. We attached p<strong>la</strong>stic tags to the bottom part of the ear using self-piercing<br />

aluminum ear tags (tag size three, National Band and Tag Co., Newport, KY). We disinfected<br />

ears by washing the site with chlorexidine v<strong>et</strong>erinary soap, rinsing with water, and applying<br />

iodine tincture before piercing. We attached tags in the carti<strong>la</strong>ginous bottom part of the ear.<br />

We attached two smaller self-piercing aluminum ear tags (tag size one, National Band and<br />

Tag Co.; hereafter ca lied mini-tags) to the upper part of the ears. We suspected they wou Id be<br />

lost less often than <strong>la</strong>rger aluminum tags bearing p<strong>la</strong>stic tags, and would therefore help to<br />

maintain i<strong>de</strong>ntification of indivi<strong>du</strong>als. We tested this hypothesis by comparing loss rate of<br />

tags versus mini-tags.<br />

Ears of juveniles were too small to support tags so we marked them using two mini-tags<br />

only. We threa<strong>de</strong>d unique combinations of colored p<strong>la</strong>stic-coated wires through mini-tags for<br />

i<strong>de</strong>ntification of juveniles at a distance. Capture techniques and immobilization proce<strong>du</strong>res<br />

were approved by the McGill Animal Care Committee (permit 4213), the Comité <strong>de</strong><br />

protection <strong>de</strong>s animaux <strong>de</strong> l'Université <strong>du</strong> Québec à Rimouski (permit CPAI2-02-06) and the<br />

Société <strong>de</strong> <strong>la</strong> Faune <strong>et</strong> <strong>de</strong>s Parcs, Gouvernement <strong>du</strong> Québec (permits 20000417-001-0 I-S-P to<br />

20060501-002-01-S-F).<br />

Co /<strong>la</strong>ring<br />

We used Lotek SMRC-5RB VHF transmitters (Lotek Wireless Inc., Newmark<strong>et</strong>, Ont.)<br />

mounted on customized, home ma<strong>de</strong> col<strong>la</strong>rs (Morin <strong>et</strong> al. 2005). We did not test back pack<br />

attachment for our radio transmitters (Craig & Keller 1986) because we believed animal<br />

movements would be less constrained by col<strong>la</strong>rs. We first tested leather col<strong>la</strong>rs modified<br />

from nylon coll ars <strong>de</strong>scribed in Griesemer <strong>et</strong> al. (1999). Following Sweitzer and Berger

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