ABSTRACTS from 16th International COnference on ... - CRRT Online

<strong>ABSTRACTS</strong> FROM 17 TH INTERNATIONAL CONFERENCE ON CRRT,
SAN DIEGO, FEB 14-17, 2012
morbidity, and assess the cost-benefit
ratio of such screening.
31. Microsample Analysis of Serum
and Urine Creatinine
Measurements
David J Askenazi, Rajesh Koralkar, John
F Moore, Stephanie Clevenger, Jon D
Sharer
University of Alabama at Birmingham
Background: Measurement of serum
creatinine in newborns can be
problematic for several reasons. In
infants born with very low birth weight,
volume loss <strong>from</strong> blood draws can
account for significant blood loss
limiting clinical and research
measurements. In addition, bilirubin
(known to be high in neonates) and
medications can interfere with creatinine
measurement by the Jaffe reaction.
Purpose: We evaluated the differences
in laboratory sampling between different
methodologies (Mass Spectrometry
(MS) vs. Jaffe). In addition, we
evaluated the ability to reliably replicate
results with variations in time <strong>from</strong> lab
draw to sample measurement [immediate
(within 2 hours), at 24 hours, and at 3
days). Finally we assessed how
measurements may be influenced by the
type of storage (Refrigeration -4 degree
C vs. Freezer -8 degree C). Methods:
We performed a prospective laboratory
analysis using whole blood and fresh
voided urine <strong>from</strong> 6 healthy adults who
had neither kidney or liver disease, and
were not taking medications known to
interfere with creatinine determination.
Each samples was processed using only
2 mcl of sample. The first method (MS)
involved tandem Mass Spectrometry
using multiple reaction monitoring and
quantitated via stable isotope dilution.
The second used Jaffe methodology on a
Beckman machine. Results: There was a
very high correlation (r= .996) between
MS and Jaffe samples and a mean %
differences between samples of 5.15 +
23.49 ng/dl. There was good correlations
and limited % difference between
measurement times and storage type in
both MS and Jaffee. (Table 1).
Immediate
vs. 24 hr
Refrigerated
Immediate
vs. 24 hr
Freezer
Immediate
vs. 3 day
Freezer
Immediate
vs. 24 hr
Refrigerated
Immediate
vs. 24 hr
Freezer
Immediate
vs. 3 day
Freezer
Method
MS
MS
MS
Jaffe
Jaffe
%
Difference
(mean ;
sd)
-5.27 +
6.67
-7.43 +
9.34
-11.36 +
9.95
2.89 +
12.68
-.24 +
2.34
Correlation
.996
.997
.999
.997
.999
Jaffe 7 + 17.15 .999
Conclusions: Although variations exist
between creatinine measurements using
Jaffe and Mass Spectrometry there is a
high degree of correlation even when
performing these tests using
microsamples. Samples were relatively
unaffected after 24 hours in a
refrigerator, freezing for 1 day or
freezing for 3 days.
32. The Effect of Poly (ADP-Ribose)
Polymerase Inhibition on
Aminoglycoside-Induced Acute
Kidney Injury in Rats
146