ABSTRACTS from 16th International COnference on ... - CRRT Online

<strong>ABSTRACTS</strong> FROM 17 TH INTERNATIONAL CONFERENCE ON CRRT,
SAN DIEGO, FEB 14-17, 2012
Alexander Biro, Hananya Vaknine,
Zipora Matas, Asora Fux, Leticia
Schreiber, Mona Boaz, Zvi Burbea, Relu
Cernes, Alexander Briliant, Ze'ev Katzir
Nephrology Institute, E Wolfson
Medical Center, Israel , Pathology
Institute, E Wolfson Medical Center,
Israel, Biochemistry Laboratory, E
Wolfson Medial Center, Israel,
Epidemiology Unit, E Wolfson Medical
Center, Israel
Background: Aminoglycosides cause
nephrotoxicity in 1-2% of patients by
generating reactive oxygen species
(ROS), leading to DNA destruction and
activation of poly(ADP-ribose)
Polymerase (PARP). The ensuing
decline in nicotinamide adenine
dinucleotide (NAD) causes diminished
cellular energetic capacity and necrotic
tubular cell death. Methods: The effect
of PARP inhibition on gentamicininduced
nephrotoxicity was studied in 2
female Wistar-Kyoto rats divided into
treatment groups: control (no treatment
or PARP-inhibitor-treated [3-amino
benzamine, 3AB]); gentamicin-treated;
and gentamicin+3AB treated. Kidney
function, protein and gentamicin levels
and urinary trypsin inhibitory activity
(TIA) were measured. Tissue
microscopic examination and
immunohistochemical study for
Proliferative Cell Nuclear Antigen
(PCNA) were determined.
Results: The following results were
obtained: Urea was 41.±5.8, 88.3±5.3
and 48.5±12.7 mg/dL in control,
gentamicin and gentamicin+3AB-treated
rats, respectively (p=.4). Proteinuria was
7.5±2.9 in controls, 41.2±18.1 in
gentamicin-treated and 17.6±13.9
mg/24-hours in gentamicin+3AB-treated
rats (p=.3). TIA was 528.75±357.9,
1365.±863.7 and 475.±194.4 inhibitory
units/day in control, gentamicin and
gentamicin+3AB-treated rats,
respectively (p=.2). The number of
macronuclei per 1mm2 was significantly
higher in gentamicin-treated rats than in
gentamicin+3AB treated rats (218±11.8
vs. 41.7±36.2. p=.4). The number of
PCNA positive nuclei was marginally
significantly higher in the gentamicintreated
rats than in gentamicin+3AB
treated rats (3585±2215.3 vs. to
626.7±236.9, p=.7). Conclusions:
The effect of PARP inhibitor on the
bactericidal activity of gentamicin was
assessed, no effect was observed.
This study illustrates that PARP
inhibitor significantly attenuates
gentamicin-induced nephrotoxicity in
rats with no effect on its bactericidal
activity.
33. Plasma NGAL Is An Early
Biomarker Of Graft Function,
Calcineurin Inhibitor
Nephrotoxicity And Tubular
Regeneration In Kidney
Transplantation From Extended
Criteria Donors
Vincenzo Cantaluppi, Michela
Tamagnone, Sergio Dellepiane, Davide
Medica, Ana M Manzione, Maria
Messina, Federico Figliolini, Luigi
Biancone, Giovanni Camussi, Giuseppe
P Segoloni
University of Turin, San Giovanni
Battista Molinette Hospital
Background: Delayed graft function
(DGF), defined as the need for dialysis
in the first week after kidney
transplantation (KT), has been
increasing for the use of kidneys <strong>from</strong>
extended criteria donors (ECD). Plasma
Neutrophil Gelatinase-Associated
Lipocalin-2 (NGAL) has been proposed
as early biomarker of DGF. Aims: The
aims of this study were to evaluate: 1)
NGAL in 5 patients in the first 24h after
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