ABSTRACTS from 16th International COnference on ... - CRRT Online

<strong>ABSTRACTS</strong> FROM 17 TH INTERNATIONAL CONFERENCE ON CRRT,
SAN DIEGO, FEB 14-17, 2012
We isolated MVs <strong>from</strong> EPC
supernatants by uktracentrifugation and
we characterized their RNA content
showing the enrichment in microRNAs
that modulate proliferation and
apoptosis. Results: After i.v. injection in
cisplatin-treated mice, MVs localized in
peritubular capillaries and tubular
epithelial cells, significantly decreased
mortality 7 days after administration and
conferred functional and morphologic
protection <strong>from</strong> AKI by enhancing
tubular proliferation and reducing
apoptosis. In surviving animals, a
preserved renal function and histology
was observed also 28 days after
injection. Evidence for a role of MVmediated
transfer of RNAs in the
renoprotective effect of MVs was
derived <strong>from</strong> the loss of MV activity
after 1) their treatment with RNase, 2)
unspecific microRNA-depletion of MVs
by EPC transfection with siRNA for
Dicer, the intracellular enzyme essential
for microRNA synthesus and 3) MV
depletion of the anti-apoptotic
microRNA miR-27a by EPC transfection
with a specific antagomiR. In vitro, we
confirmed the role of miR-27a in the
anti-apoptotic effect of MVs in cisplatintreated
human tubular epithelial cells.
Indeed, MVs derived <strong>from</strong> EPCs
significantly reduced apoptosis through
the down-regulation of the death
receptor Fas (CD95), of the
mitochondrial molecules Bcl-XL/Bcl-2
and of caspase-3, -8 and -9 activation.
These effects were not observed after
miR-27a depletion. Conclusions: MVs
derived <strong>from</strong> EPCs protected <strong>from</strong>
cisplatin-induced AKI by delivering
their RNA content. The miRNA cargo of
MVs and in particular miR-27a
contributed to reprogramming cisplatininjured
tubular epithelial cells toward a
regenerative program, inhibiting the
death receptor/mitochondrial apopototic
pathways.
35. Classification Of AKI Using P-
Rifle Score In A Picu In
Fundación Valle Del Lili, Cali,
Colombia
Gastón Castillo, Angie Cañas, María del
Pilar Duque, Fernando Bermúdez,
Eliana Manzi, Teresa Agudelo, Jaime
Restrepo, Magda Cepeda
Fundación Valle del Lili
Introduction and Aim: Acute Kidney
Injury (AKI)\'s incidence in PICU
patient worsen mortality. We used the p-
RIFLE score to estimate the incidence of
AKI in children of PICU in an institute
in of fourth level in Cali. Methods:
Prospective study of patients
hospitalized in PICU between
september/29 and august/211, with AKI,
in whom the p-RIFLE score was applied.
Results: Among 1891 patients registered
in PICU, 3.86% presented AKI. Half
were under 24 month age (p25-p75: 6-
18), and 58% were male. At 24 and 72
hours of admission, 43% and 66% of
patients presented Failure, respectively.
The principal admission diagnosis were
cardiovascular (34%) and infectious
(18%). 32 (44%) dead. Mortality by p-
RIFLE in 24hrs is similar across strata,
while at 72hrs Failure was higher. The
relation in mortality was invested for
patients classified as Failure among 24
and 72 hrs, vs. observed in Risk and
Injury (Graphic). The association of
mortality with p-RIFLE at 72hrs was
significant (p=.), while at 24hrs did not
(p=.712). 4% of patients were classified
as very high of mortality by PRISM, but
was not associated with mortality
(p=.455). 39 (53%) of patients required
RRT, but was not associated neither
mortality (p=.368) nor worsening of
RIFLe (p=.99). Conclusions: The use of
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