The genus Cladosporium and similar dematiaceous ... - CBS - KNAW
The genus Cladosporium and similar dematiaceous ... - CBS - KNAW
The genus Cladosporium and similar dematiaceous ... - CBS - KNAW
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Schubert et al.<br />
Fig. 41. <strong>Cladosporium</strong> subtilissimum (<strong>CBS</strong> 113754). A–C. Macronematous conidiophores. D. Conidial chain. E. Micronematous conidiophore. F–G. Conidia. Scale bars = 10<br />
µm.<br />
<strong>Cladosporium</strong> subtilissimum K. Schub., Dugan, Crous & U.<br />
Braun, sp. nov. MycoBank MB504580. Figs 40–42.<br />
Etymology: Refers to its narrow conidiophores <strong>and</strong> conidia.<br />
Differt a Cladosporio cladosporioide conidiophoris et conidiis semper asperulatis ad<br />
verruculosis, conidiis 0–1(–2)-septatis.<br />
Mycelium unbranched or sparingly branched, 1–5 µm wide, septate,<br />
without swellings <strong>and</strong> constrictions, hyaline to subhyaline or pale<br />
brown, smooth to minutely verruculose, walls unthickened or almost<br />
so, protoplasm somewhat guttulate or granular. Conidiophores<br />
macronematous <strong>and</strong> micronematous, arising laterally from<br />
plagiotropous hyphae or terminally from ascending hyphae, erect,<br />
straight to slightly flexuous, filiform to cylindrical-oblong, nonnodulose,<br />
sometimes geniculate towards the apex, unbranched or<br />
once branched, branches short to somewhat longer, usually formed<br />
below a septum, sometimes only short, denticle-like or conical,<br />
25–140 × 2–4 µm, 0–4-septate, not constricted at the septa,<br />
subhyaline to pale brown, almost smooth, minutely verruculose<br />
to verruculose, sometimes irregularly rough-walled in the lower<br />
part, walls unthickened or slightly thickened, protoplasm guttulate<br />
or somewhat granular. Conidiogenous cells integrated, terminal<br />
or pleurogenous, sometimes also intercalary, filiform to narrowly<br />
cylindrical, non-nodulose, sometimes geniculate, 14–57 µm long,<br />
with usually sympodial clusters of pronounced conidiogenous loci<br />
at the apex or on a lower level, denticle-like or situated on short<br />
lateral prolongations, up to five loci, intercalary conidiogenous<br />
cells usually with a short denticle-like lateral outgrowth below a<br />
septum, protuberant, denticulate, somewhat truncate, 1.2–2 µm<br />
diam, thickened <strong>and</strong> darkened-refractive. Ramoconidia sometimes<br />
occurring, conidiogenous cells seceding at one of the upper septa<br />
of the conidiophore <strong>and</strong> behaving like conidia, filiform or cylindrical,<br />
20–40(–55) µm long, 1.5–4 µm wide, 0–1-septate, concolorous<br />
with conidiophores, not attenuated towards apex <strong>and</strong> base, base<br />
broadly truncate, non-cladosporioid, without any dome <strong>and</strong> raised<br />
rim, 2–3.5 µm wide, neither thickened nor darkened, sometimes<br />
slightly refractive. Conidia catenate, in branched chains, up to 12<br />
or even more in a chain, straight, small terminal conidia numerous,<br />
subglobose, narrowly obovoid, limoniform or fusiform, 4–9 × 2–3.5<br />
µm [av. ± SD, 6.4 (± 1.5) × 2.8 (± 0.4) µm], with up to three distal<br />
scars, aseptate, hila (0.5–)0.8–1 µm diam, intercalary conidia<br />
narrowly ellipsoid, fusiform to subcylindrical, 9–18 × 3–4(–6) µm<br />
[av. ± SD, 13.0 (± 2.5) × 3.8 (± 0.3) µm], 0(–1)-septate, hila 1–1.2(–<br />
1.8) µm diam, with up to four distal scars, secondary ramoconidia<br />
ellipsoid, fusiform or subcylindrical, (13–)17–32(–37) × 3–5(–6) µm<br />
[av. ± SD, 21.4 (± 4.4) × 4.1 (± 0.5) µm], 0–1(–2)-septate, septum<br />
median or somewhat in the lower half, usually not constricted at<br />
the septa, with up to six distal hila crowded at the apex, hila (1.2–)<br />
1.5–2(–2.5) µm diam, apex often somewhat laterally enlarged<br />
or prolonged with hila crowded there, very pale or pale brown or<br />
olivaceous-brown, minutely verruculose to verruculose (granulate<br />
under SEM), walls unthickened or only slightly thickened, often<br />
slightly attenuated towards apex <strong>and</strong> base, protoplasm often<br />
guttulate or granular, hila protuberant, denticulate, (0.5–)0.8–2(–<br />
2.2) µm diam, thickened <strong>and</strong> darkened-refractive; microcyclic<br />
conidiogenesis occasionally observed.<br />
Cultural characteristics: Colonies on PDA attaining 24 mm diam<br />
after 14 d at 25 °C, grey-olivaceous to olivaceous, olivaceousgrey,<br />
iron-grey or olivaceous-black reverse, velvety, margin<br />
regular, entire edge, white or pale greenish olivaceous, glabrous<br />
to feathery, aerial mycelium sparse, only few areas with abundant<br />
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