Medical Hydrology and Balneology: Environmental Aspects

Balnea
2012, núm. 6, 200-201
Medical Hydrology and Balneology: Environmental Aspects
to a reduction of iNOS in the cells. This decrease was down to 5% positivity for
NaHS (similar for all the concentrations) and down to approximately 1% for all
GYY4137 concentrations except for 50 µM which did not cause a reduction.
Regarding the production of ROS, basal conditions had approximately 9% positivity.
Addition of 50 µM and 100 µM GYY4137 resulted in a reduction of ROS
down to 0.11% and 0.25% positivity, respectively; however, for 1000 µM positivity
was again increased up to 9.95%. For NaHS, ROS positivity was reduced to 7.54%
and 5% for 500 and 1000 µM, respectively. We also saw a concentration-dependent
increase in the expression of SOD2 mRNA in the IL-1β stimulated cells treated
with the S-containing compounds, this may in part account for the observed decrease
in ROS.
Conclusions
These results indicate that the hydrogen sulfide, the main component of sulfur
waters, may have anti-oxidants and anti-inflammatory properties. Therefore, therapies
that make use of these waters may represent promising alternatives to explore
for the treatment of rheumatic diseases, such as osteoarthritis
Keywords: Sulfur Water; Chondrocytes; Osteoarthritis; Hydrogen Sulphide; Antioxidant
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