Medical Hydrology and Balneology: Environmental Aspects

Evidence of hypocholesterolemic effect of calcic
magnesic sulphated bicarbonate mineral water
(Bonneval) in cholesterol-rich diet fed rat
Cambar J (1) , Vindigni JD (2) , Dubourg K (1)
(1) Institut du thermalisme, Dax, France
(2) Bonneval Emergence, Bordeaux, France
jean.cambar@u-bordeaux2.fr
Introduction and Objectives
The purpose of this study was to evaluate the ability of a calcic magnesic sulphated
mineral water (CMSMW) issued from Bonneval Edelweiss spring to reduce
plasma triglycerides and cholesterol in rats fed a cholesterol-rich diet.
Materials and Methods
40 male Wistar rats are distributed in 4 lots of 10 each: lot 1 (1% cholesterol-rich
diet with tap water drinking), lot 2 (1% cholesterol-rich diet with Bonneval water
drinking), lot 3 (2% cholesterol-rich diet with tap water drinking and lot 4 (2%
cholesterol-rich diet with Bonneval water drinking). Rats were fed with two cholesterol
diets UAR- 214B (1 and 2% cholesterol). Plasma samples were assessed at J0
and every week (D7, D14 and D21) for triglycerides, total cholesterol, HDL and
VDL cholesterol determination. Weight estimation war realized every week. At
D21, animals were sacrificed and total liver weight estimated.
Results
Cholesterol-rich diet shows well a dramatic increase in plasma cholesterol in all
lots with a large difference between 1 and 2% cholesterol rich diets. For example,
for lot 1 (1% diet), at D0: 0,96 g/l and at D21: 2,99; for lot 3 (2% diet), at D0: 0,97
g/l and at D21: and 4,70 g/l. No significant differences in total animal weight
changes can be noted between lots 1 and 2 and between lots 3 and 4 during the
experiment and at D21. Macroscopic aspect and total weight are similar in all 4 lots.
Significant results of Bonneval water efficacy can be observed only in lot 2 (vs lot
1) with a 1% cholesterol diet. The liver induced damages and plasma lipids level are
too severe in lot 3 and 4 with 2% cholesterol rich diet. After 7 days of treatment
with Bonneval water, a highly significant decrease in plasma triglycerides (2.19 g/l
vs. 1.53 g/l, p