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For more information on Biology see the ENVIRONMENTAL<br />

ENGINEERING section.<br />

CELLULAR BIOLOGY<br />

♦<br />

SEX PILUS<br />

LYSOSOME<br />

FLAGELLA<br />

COMMON PILUS<br />

ANIMAL<br />

3µm<br />

1µm<br />

RIBOSOMES<br />

PEPTIDOGLYCAN<br />

CYTOPLASMIC<br />

CHROMOSOME<br />

INNER OR CYTOPLASMIC<br />

MEMBRANE<br />

10µm 20µm<br />

STARCH GRANULES<br />

MITOCHONDRIA<br />

PLASMA<br />

MEMBRANE<br />

NUCLEUS<br />

ENDOPLASMIC<br />

RETICULUM<br />

GOLGI<br />

COMPLEX<br />

ROUGH<br />

ENDOPLASMIC<br />

RETICULUM<br />

CHLOROPLAST<br />

TONOPLAST<br />

CENTRAL<br />

VACUOLE<br />

PLANT<br />

• Primary subdivisions <strong>of</strong> biological organisms<br />

Group<br />

Eucaryotes<br />

Eubacteria<br />

Archaebacteria<br />

Eucaryotic<br />

Multicellular; extensive<br />

differentiation <strong>of</strong> cells<br />

and tissues<br />

Unicellular, coenocytic or<br />

mycelial; little or no<br />

tissue differentiation<br />

Procaryotic Cell chemistry similar to<br />

eucaryotes<br />

Procaryotic Distinctive cell chemistry<br />

PERIPLASMIC<br />

SPACE<br />

OUTER<br />

MEMBRANE<br />

Cell<br />

structure Properties Constituent groups<br />

BIOLOGY<br />

CELL WALL<br />

Plants (seed plants, ferns,<br />

mosses)<br />

Animals (vertebrates,<br />

invertebrates)<br />

Protists (algae, fungi,<br />

protozoa)<br />

Most bacteria<br />

Methanogens, halophiles,<br />

thermoacidophiles<br />

74<br />

� Organismal Growth in Batch Culture<br />

BACTERIAL NUMBERS<br />

10 6<br />

10 5<br />

10 4<br />

10 3<br />

LAG<br />

PHASE<br />

STATIONARY<br />

PHASE<br />

LOG GROWTH PHASE<br />

ACCELERATED GROWTH<br />

PHASE<br />

DEATH<br />

PHASE<br />

5 10 15 20 25 30 35 40 45 50 55 60<br />

TIME (h)<br />

Exponential (log) growth with constant specific growth<br />

rate, µ<br />

⎛1⎞⎛dx⎞ µ= ⎜<br />

⎝<br />

⎟⎜<br />

⎠⎝<br />

⎟<br />

x dt⎠<br />

where,<br />

x = the cell/organism number or cell/organism<br />

t =<br />

concentration<br />

time (hr)<br />

µ = the specific growth rate (time –1 ) while in the<br />

exponential growth phase.<br />

Logistic Growth–Batch Growth including initial into<br />

stationary phase<br />

dx ⎛ x ⎞<br />

= kx⎜1−<br />

⎟<br />

dt ⎝ x∞ ⎠<br />

kt<br />

xoe<br />

x =<br />

xo<br />

1− 1−e x ∞<br />

kt ( )<br />

where,<br />

k = logistic growth constant (h –1 ),<br />

xo = initial concentration (g/l)<br />

x∞ = carrying capacity (g/l).<br />

♦ Shuler, Michael L., & Fikret Kargi, Bioprocess Engineering Basic Concepts, Prentice Hall PTR, New<br />

Jersey, 1992.<br />

• Stanier, Roger; Adelberg, Edward A; Wheelis, Mark L; Decastells; Painter, Page R; Ingraham, John<br />

L; The Microbial World, 5th ed., 1986. Reprinted by permission <strong>of</strong> Pearson Education, Inc., Upper<br />

Saddle River, NJ.<br />

� Davis, M.L., Principles <strong>of</strong> Environmental Engineering, McGraw-Hill, New York, 2004. Used with<br />

permission.

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