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till the growtli of bacterial cells \Val complet~ly tcrn~in~ted. Tlic cultl~red explaits were
maintamed in the inon-select~on induction nlrdli~nl tllal d~d 1101 co~lsibt of kanil~nycin, for
clbout 3 to 4 diiys. TIIC l'\jild~~ts \\ere tlle~l trG~nsl;.rrcd lo tile MS IIIC~IIIII~
co~lti~i~ling 25 III~IL
k;~noniyc~n i111d III~II~~~IIIIL'~~ tbr OIIC \\eck. TIICII the c\l>l;i~~t> \\ere CLIIIII~C~ 1111hlS III~~~~IIII
COIII;IIIIIII~
50 1113 L /\~II~~III~!C~II ;III~\\ere I~I;IIIII;IIIIC~ liir ;10o11t 7 10 lU d,iy\. 'rile slloot buds
~III~VIII~ CIS bi111c11ca \\ere ~;~rc(i~ll! ~C/I;II.:IIC~ liulil tl~e CUI~IC~OII~;III \\ 1111 >OIC bi15;ll ci~lliia
IIII~IC~ i~~lJ IIIc! !\CIS ~ri~~~>li'rrciI 10 111c 51111111 ~I~II~~III(III IIIC~~I~IIII C~IIII~IIIIIII~ 5 11hI 2-11) :III~ 2
pb1 ~III~IIII (SEhll) i111ti 75 1n1g'L Lc~~~,~~li!
CII~ Ali~~r LILI{I IU ~I,I)\ 111~ICIII~;IIC~ :IS \$,ell 21s 111c
~~~i-clo~l@,~tcd sllootb iicre tr;~~~sli.rretI to tile slioot clongatioll iniedii~n~ 111.it ~olltaincd MS \vilh
2 pM GA, (SFhl2) ,~nd IUU m$L L;~I~;~III~~III. 'She ~~n-lr;~~~slurn~cd >11oo1~ICLICIIC~ i11 every
sli~gc oi'tllc III~IIC~I[III
,III~ ~ICIII~~II~UII Tile ~CICCIC~ \IIUUI)
III~II \\CIC g111\1111g II~;II~IIII~ \vcrc
carci'i~lly separntcd liom lllc elotipotii~g slioot builcll :lilti irere cuhl~rcd ill the root inducrlon
II~~~~IIIIII \\IIIc~~\\;I,
~~re~i,~red \VII~IOLII
;I~~III~ tl~c \CICCIIUII ;IIII~~~~IIC ~~III~IIII~CII~ i~\
kil~~ili~iycin
\ 11u