Download (26Mb) - OAR@ICRISAT

phase (Scarpa et a]., 1992). Direct and indirect nlultlple shoot regeneration was reported in
the winged bean, Psophocorprrs tetrugo~~olohris. Shoot tip, epicotyl, hypocotyl and
internode explants were cultured on Ms rnedia supplelnented wit11 different concentrations
and combinations of BAP, NAA and IAA. Plant regeneratton was acllleved from internode
and hypocotyl explants via direct orga~iogenesis and fronl internode and ep~cotyl explants
via indirect orgallogenesis drpend~ng on tllc gsuwti~ regulators (A~~julll;iilara et sl., IYc)X).
Callus derived shoot regeneration was achieved from root explall~s of Lutilyr~a sor~vus by
using various concentrations of kinetin and rootillg was done on IBA (Roy et al., 1992).
Macrufiiiurn uirupurpureuni is a ~uodei plant wit11 broad synlbiont range fbr ~lodulatlon.
First report of in vitro plant regeneration 111 th~species by using hypocotyls expiants was
reported by Ezura et al. (2000). A novel rnethod of ind~rect organogenic regeneration was
developed for Pisuni soiivirn~ using thin cell layer segments of nodal explants I'roni which
leaflets and axillary buds were removed (h'aue1.b~ et al., 1991). Indirect regeneration of
shoots via callus phase was obtained In paature legu~lles Lolo~~urlls Darrie~ii (Bovo et al.,
1986), Cer~iruse~rla brusilionurr~ (Angclo~u et al., 1YY2), Desr~totliur~i uJJine and D.
unciirofu~r~ (Iley and Mroginski, 1997). Various explants like hypocotyls, root and leaves
of pasture legume Slyloso~rfhrs g~lyane~~~is were used to regenerate shoots via callus pha~e
(Meijer and Broughton, 198 1).
2.2.2.2 So~natic enlbryogr~~esis
Five species of Metiicagu, M. ciliuris, M. ~r~erc.~, M. orbicuiaris, M. polyniorpha
and M, truncuiulo were assessed for somatic en~bryo induction with 2,4-D. Incorporation
of PEG resulted in better maturation of the embryos (lantcheva et al., 2001). Callus was
induced from stloot-tip cult~ires of eight species of Tri/diurn and whole plants were