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and nprII for selection. PCR and Soutllern analyses confinned the integration of transgene
(Venkatachalam et al., 1998). Transformation of A. hjyogaea was also done using the
somatic embryogenesis pathway. The cotyledons were co-cultivated with A. rumefaciens
strain LBA4404 containing uidA and 11pill genes. Somatic en~bryos were ~nduced with
NAA and BAP and later regenerated into whole plants with a transformation frequency of
47% (Vcnkatachalam et al., 2000). Embryo axis explants were uaed for thc
Agro6ucie1~iiii1i-ri1ed1ated transform;~tion in /lriiciri;, I~ypogcieu (McKenlly et al., 1995). In
vitro grown seedlings of Crijoirrrs crijait were inoculated with three types of wild strains of
Agrobncirriu~ir A281, A6 and T37 and cultivar-Agrobacleriiiir~ strarn specilic induct~on
tumors was found (Rathore and Chaod, 1997). Lcaf disks of pigeon pea cv. ICPI5164 were
transfor~iied by il iur~iejiciens strain LBA4404 plasniid pBAL2 carrying kanumycin
resistance and GUS reporter genes under tile control of tile 35s promoter. The optimul~i
period of coc~ilt~v;i[ion was 4 days, giving 47.8% tronsfor~ned calluscs (Arundhati, 1999).
Tr;~nslbrn~alion of pigconpea wila acli~evcd LISII~~
A. r~i~rlefu!/a(.if~t~ strain GV2260,
containing the construct of isolated cowpea proteinase inhib~tor gene, pCPI. The gene was
dr~ven by CaMV 35s promoter containing kanamycin resistance as plant selection marker.
hlolecular analysis of tile putative transibrniatits was done by Northern blotting technique
(Lawrence and Kuundal, 2001). /Igrobocleriiii11-n1ed1ated transformation of Vigi~u
sesquipeiiuiis was achieved using cotyledonary node explant, where 2% of the shoots
showed integration of riprII, phosphinothr~cin acetyl transferase bur) and uidA genes.
Integration was confirmed using GUS histo-chemical assay and Southern blot analys~s
(Ignasimuthu, 2000). Hypocotyl and primary Icaves excised from 2-day-old in vltro grown
mungbean (Vigrla rudiata) were used for tlle transfomiation studies. This particular gram