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Rand, G.M. (Ed.) (1995) Ftordir~~rc~~r~ls q/'Aiitr'i/tc~ Tocivolog~: Wrcrs, E~rviro~r~~roltrri Fare o11d Risk Assess~rie~~r, pp. 1 125 Rao, B.G., (1989) Effects of constiturtils 2nd tlieir coticc~i~r,~~iuiis ill Iiulrlclit 1iledi:l 011 callustng lion1 chickpe;~ hypocotyls, Lc~gr111rc' Hnriiri11. I!: 186 18s. Rae, B.G., Cl~opra, V.L., (1987) Genotypic atid e\pl;itit diffcrrticea 111 c;illus 1111t1;1[ioti 2nd ~niil~literla~~cr 111 chickpe;~, I~~rrr~~i~~~ro~iirl ('iiic hprii ,Yr~~.\/~~rci~~.. 17 10- I? Icao, B G.. Choprtr, V.L , (1989) Rege~ieralioll ill cli~ckpe;~ (('i1.r~ irririi~i~r~~i L ) 111rougli somatic enibryogenesis, Jorrr~tul oJ Plir~tr PIri,~iolog,, 134: 637-638. 11atliore, R.S., Sillgll, N.K., Garg, G.K., Clund, L., (2000) llegcr~eratiuti ol'~>l;lnts from callus tissue of AL'SCII~IIUIII~II~ spp. (Leg~~tiirtios;~~), JUII~IIII~ (I/ PI NruIo,~:y, 27. 81-84. Rathore. R.S., Ch;lnd, L., (1997) In vitro tr;l~lsfbr~iiolio~i ol' piyco~ipc:~ gcnulypes by wild stralris of AgroDocrerirr~~i rrr~~icfiicie~rs, I~~/er~rcirto~rtrl Cl~ic'k/~cci o~ril /jrgro~rl)ro Neir~ic//el., 4:38-39. I?aupl), M.J., Koehler, C.S., Uacidsoli. J.A., (1992) Adv;rllccb 111 ~l~iplcn~cnt~ng integrated pest rnalingenient Ibr woody laiidacapc pl;~llls. A111rrtu1 /(ci'rci~' 1!/ E~iro~~rulugy, 37: 56 1-585. Ila~en, J.A., (1986) B~ucllem~cal dispo\;ll ul' chccss Ilt 111 glowllig pl;ililb'!, New Ph)~roIogiii, 104. 175 - !U(I Kazdan, M.K., Cocking, E.C., Po~er, J.B., (IYXU) Callua rcgcrleratlurl li-otil nirsopliyll p~.otoplaita of sweet pca (Lor/r~~ru\ odorirru.~ L.), Zur.scltriji jiter PJla~~zr~~/i/~vsioiogir, 96: 18 1 - 183.
Rech, E.L., Golds. T.J., Hammatt. N.. Mulliga~i, B.J., Dave), h.1.R.. (1988) Agrobocreriu~~l rhrroge~les lnediated trn~isfori~~atioii of the \\,~ld soybeans (;11,~,iri~~ ca~iescens and G. cic~~iciesritio: producl~on of tmllsgenlc pluilts of (; c.o~resct~rts.. Jocrrnni o/'E.rprri/~ti.~lrtrl Ilulo~t,,, 39: 1275- 12x5 Reddy, L.R.. Reddy, G.M., (1993) Faclors :~ll;.ct~ng dlrccr soill;itlc cii~bryogeiles~s :111d p1a11t regc~~e~ilioii 111 grou11d11~11, .4r11ci1i.s Ii)pug~i~,c~ L, /III/~UII JOIII.IIII/ I,\ EA/I~I.IIII~II/~I/ Uioiog): 3 1: 57-60 Kepkov:~, J., Nedbalkova, B , I-iolrib, J . (1991) Ilegunel-;it~uil of pl,~nLa li.o~il /ygo~lc embryos after lnterspecilic Ihybr~dizalloll willli~~ tllc ~CIIUS I).!/uli~r~~i ;111d electrophoretic evaluario~i of hybrids. Scir~rlijic. S/iirii~,r OS6111 Koi,iirc.h I~i.\~iiiirc jbr Fu(1cier Pla~r/s, 12: 7-14, Iley, H.Y., Mroginski, L.A., (1996) Regc~lc'itio~l ol' ~pl:liils lioln c;~llur t~hrut~c ol IIJIIIIIIL spp. (Legurilillosne), i'lii~~t ('i,il, %~AIIL. IIIII~ 0rgo11 ('rtl/rir
Page 1:
GENETIC TRANSFORMATION OF Cicer ari
Page 4 and 5:
Certificate Ger/$ed /La/ /Ae eldire
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I deem il as a great pleastire to e
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CONTENTS ABBREVIAI'IONS SUMMARY LIS
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3.2.2.3 Shoot elongat~on 3.2.2.4 Ro
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ABBREVIATIONS 2-il' 2-isopci~~cnpl
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frequency was observed wit11 2,4,5-
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in vitro grown plants w;is ocli~eve
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LIST OF TABLES Table 3.1 Pr~~iier c
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Table 4.1 1 Effect ul'age uf lhe ex
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Figure 4.1 A - D inducl~on of sonla
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Figure 4.9 A - C Alternative llleti
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Figure 4.15 A, B Restriction analys
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Figure 4.20 A, B DNA profile of gen
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1'1.0 INTRODUCTION L~fe is the ter~
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them. About 10% of the Earth's land
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observations GkI Shull's experiment
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(Nene and Haware, 1980). The legume
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jf.0 REVIEW OF LITERATURE Large par
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plants from them enlhuscd scientist
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introduction into protoplasts using
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evident by 1983 that the Agrobacter
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The above promoters were found to b
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gained an illstant popi~larity owi~
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legume crop species. Cotyledon expl
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1994). Clonal propagation of FI int
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were induced to for111 callus when
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' the ernblyos into plantlets on MS
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and nprII for selection. PCR and So
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Agrobacleriuiii and ill otlier expe
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ioteclinologicsl i~iiprovemel~t of
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combinat~ons and concentrdtions of
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egenerated via somatic eliibryogene
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egenerat~on from protoplasts and ca
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transformants were identified by sy
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urgent econoniical control measures
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applications of traps. Technically
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in the laboratory and releasing tl~
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have been exhaustively reviewd (Dai
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larvae of certaln Insects were unab
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3.1 Pl:~nt 111nteri:il sllrl cultur
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0.5. 2.0 and 5.0 pM were added. 111
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Lec!/i?is: Young anci juve~iile lea
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AM3 explant: This was a by-product
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PM) 2nd GAJ (2 5 PM), eltller singl
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of the plastic bag was removed rwlc
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- . -- laopropyl alcoliol ; Absolul
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The sl~des were Illell muiaited and
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6. The bupcniatant \\,I,, t,ikcli i
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5. Microcal~lers were allowed to se
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till the growtli of bacterial cells
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;I! 37 'C for 3-24 Iiours 111 d;irL
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PCN for 111cl.4 ge!iiJ: I'C'I< tbr
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4. Tliu pel \\as \r;~alicd 3 ti~iir
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4.0 RESULTS $loaf of tllc c\l~cr~~l
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These combi~iatiori sl~owed liiorc
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egeneratloll \!;IS dli'licull ;~nti
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decreased a[ cllkalinc pH \+li~le 1
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either side of'bws;tl portiol~ oill
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IBA sl~owed bro\\~~i~~g i~lld de:~l
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humidity uf 50% 111 the dayrinie a1
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4.2 Histological studies ~II nlultl
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sliowetl no liirtlie~ yrowlli Tllc
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Conlirmntioll of plllati!e t~.~ll~a
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5.0 DISCUSSION During tile la31 dcc
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leatlets (Dilieshkuniar er al.. 109
Page 136 and 137: used with an add111ou;ll care :u~d
Page 138 and 139: tissue to regenerate illto lesser i
Page 140 and 141: prololigcd culture on TUL sho\\~d 1
Page 142 and 143: exceed 5 cni Icngtl~. Loiigcr shoot
Page 144 and 145: stem and lravca Secol~d;lrq ~'IIICI
Page 146 and 147: arnplitication of the sopccIi\i. 90
Page 148 and 149: Explant Preparation -Geminate seeds
Page 150 and 151: 6.0 REFERENCES Adkins, A.L.. Godwin
Page 152 and 153: Avenido, R.A., Hattori, I
Page 154 and 155: Benfey, P.N., Chua, N.1-I., Ilcgiii
Page 156 and 157: Cnrvalho. IC1.H C dc , Uui Vat1 LC
Page 158 and 159: Chu, P.W.G., Andersoli. B.J., Khan.
Page 160 and 161: Deblaere, R., Reyneerts, A., Hotte,
Page 162 and 163: Drun~mond, M.H., Gordoil. M.P., Nes
Page 164 and 165: Foster, J.G., Coulombe, B.A., Van S
Page 166 and 167: Gill, R., Saxstla, I'.K., (1992) Di
Page 168 and 169: Hess, D., (1970) Versueche zur tran
Page 170 and 171: Hopkinson, M.J. ;md Collins, I1.M.,
Page 172 and 173: Karthikeyan, A.S., Sarina, K.S., Ve
Page 174 and 175: Krens, F.A., Molendijk, L., L., Wul
Page 176 and 177: Li, Z.Y., Tanner, G.J., Larkin. P.J
Page 178 and 179: Maxan~, A.M., Gilbert, W., (1977) A
Page 180 and 181: field conditions. Procc.rdbigs o/Na
Page 182 and 183: Ohara, A., Akasako, Y., Daimon, H.,
Page 184 and 185: llechrnlics sur les prnidilies des
Page 188 and 189: Richardson, M.J., (199 I ) Seed sto
Page 190 and 191: Sarria, R., Calderon, A., Thro, A.M
Page 192 and 193: Shri, P.V., Davls, T.M., (1992) Zen
Page 194 and 195: Tegeder, M. Koli~i, I-I., Nibbbe, M
Page 196 and 197: Venkatachalam, P., Klshor, P.B.K.,
Page 198 and 199: Winans, S.C., (1992) Tiyo way chemi
Page 200 and 201: 1 APPENDIX 1. hIURASRICE AND SI(SO)
Page 203 and 204: Table 4.1 Induction of sorrlalic er
Page 205 and 206: Table 4.3 111ducti011 of en~bryos O
Page 208 and 209: 'Table 4.6 Dificrent combinations o
Page 210 and 211: Table 4.8 Effect oI'TDZ, 2-iP :~nd
Page 212 and 213: Table 4.10 Effect of i~lclusio~~ or
Page 214 and 215: Table 4.12 Induction of multiple sh
Page 216 and 217: Table 4.14 Effect of media constitu
Page 218: Figure 3.1 Dii~gralll~l~i~tic rcpre
Page 222: Figure 3.3 A - C Diagrn~nn~atic rep
Page 225 and 226: I . Nhu 1 51 ?C,%- .pal5125 &pa1512
Page 228: I
Page 232: Induction of somatic embryos from m
Page 238:
I.iy111. J.5 SI:i;i.\ iil ~ ~ l ~ ~
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IIistologic:~l stutlic\ of dcveiopr
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Rc\[ricfiot~ ;111;1l)si\ ur ill? ~
Page 262:
I'C'R :~llipLiiir:iriuil 01'700 bl)
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Fixi1r.c 17 \r:15 -l)K,.\ ;!lid J"
Page 272:
1:igurr 4.22 Sui~lllcrn :III:II!s~$
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