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These combi~iatiori sl~owed liiorc cnllua cu~iipored to tlir ?.4.S-l'-co1itai1ii1iy media where
the embryos originated liul~i tl~ callus III~IS~CII~. TIic iii~~iibcr ~I'expla~~ts respo~idi~~p was
s~m~lar but tllc aicrage I~LIIII~C~ ut'e~~ibryu~ per exl>Ialit \\.;IS
cu~iil);~~.;~~i\eI! Io\ver tl1i111 the
abo\e-nient1u11cd JELl aeiies ol'~~icd~;~. Tlic c~iib~qus :~l>l>c;~rcd I~~rgcr tliii~~ tlic UII~S III JEM
scrle,
;III~ tiley l;~cbctl ;illy atalhs. Fig. 4.2 sllu\rs 111~luction ol' e~~~bryos fro111 111;lturc
e111bri.o ;ial:, cul)l;~~ir. Ucs~ rcsl)o~idlng COII~~III~I~IUII \\,IS
?O 11hl 2,411 w~tli 2 1lM kkieti~i
tli;ir ilidi~ced 9.3 c~iibryos per c\pl;iiit No sccontl;~ry enlbryos ~ c ubscrvetl ~ e e\en VII
prolonged CLIIILISC ul' tlic c\pl;i~it\ 111 11ic b~~~lie 1iiec11111ii \\li~Ic tl~c 111d11ccd c~iibryos
gfiidunlly entered c;~lli~s pli;isc
V;il.~oua otllcr ~ hpl~~iti s~lcli :la 1);1r1:,
o1'111:1lurc e~iibryu ~1x15 (~>lu~iii~l~, radicle. side
;irlns ;lntl ~ii~tltllc purrion). Icaflcl>, stelii aeglnclits ;uid root acg~ilcl~t\ wcrc ci~ll~~rctl on bcst
CII~~S)O~~IIIC COIII~II~~IIIUII ui'JLhl20 rIi;~t CUIIL;I~I~C~ I0 piVl ?.1,5-T ;III~ 2 11M ~IIIC~III, I'hc
results ;ire allo\\li 111 rlic 1;lblc 3.5. I'lu~nc~le iiolil 1ii;iture c~iibryo ;IXIS
;111tlcaflel:, g;~vc bcst
c~iibryi~ ~iiJui.t~o~i li.cij~lcllc) ol'70 ;IIILI JO'% I~II~SCII\CI~. All IC UI~ICI CSPI;II~~~ CKCCP~
r.id~clc ti.uni c~iibryu ,isls sliu\rccl luglicr iiccltlc~icy of II~~LICII~II \+liere ;I t~~iic tlepc~idc~~t
~nducuo~i \ids obhcric~l. 111 ge~icl.;il, clilbryu ~~~~luctioli \V,I~ observed u~tliin 4 to 5 weeks
and s~icli phcnornc~lui~ \\;I, obser\cd ii~tli iiinlllre enibryu ;ix~s dc~.~vcd cxpla~its and
le:111c1h IIo\+cYcI.. at~111 ii~icl 1.001 \egIIiCliti sllu\\i'd III~IICIIUI~ 011 ;I 1)ruIu11gcd CUI~LI~C on tlic
:,;i~iic 1nedi~1111 Iur ;~buut X lo 9 \\ccks. Tlic cnibryo\ ;~ppcared a~ni~l:ir tu tllc ones on cliibryo
nsls and leaflet explnl~ls. Tile epldernial I;iyer ol' SI~III
and root scgmcnts broke opcn into
crevasses and embryos apl)c:~rcd orig~~ii~ti~ig fro111 tlic ililicr tissue.