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Figure 4.18 A, B
PCR arnplificatio~l of 1.2 kb fragment of tridA gene from genomic DNA samples of
To generation putative trat~sgenic plants tra~isfonl~ed with pHS723:Bt and pHS737:SBTI
vectors via Agrobocferiir~~t-inediatcd transfor~~~at~o~~. A. GUS-PCR analysts of plants
transfom~ed wit11 pHS723:Bt vector. Lanes I to I0 \\,ere added \vitIl putntivc tratisfor~ll;i~~t
samples, while lane I I \\as positive control and lane 12 Ineg;ltlve control. Satllple addcd
In lane 13 was -DNA, h DNA-UbfE II m;~rket was added in tlie lane 14. U. GUS-PC'K
analysis or plants tra~~slbrlncd wttl~ pHS737:SB'l'l. Lanes I
to 9 wcre putarivu
transformant samples, wliile lane 10 and I1 were positive co~ltrols of plasmid
pHS737:SBTI and lane 12 was negative conlrul. San111le added in lane 13 was -Dh'A. h
DNA-UsfE II ninrket- was added ill llle lane 14.
Figure 4.19 A, B
PCR amplification of SBTI end BfCrj,I/IL gcncs fro111 ge110111ic DNhs of ptllative
transforn~ants at To gene ratio^^ transformed vin Agrobacfn.ir~t~~-t~~cdi~~ted transforlnation.
A. PCR analysis of plants rr:~nsfornied with pHS737:SBTI veclo~.. Ln~li: 1 tl~ruugh lane 9
are putaive transfun~la~lt s;~tilples sliu~viiig an~pliiic;itio~~ ufJ97 bp fr:~gment. Ncgi~tive
conrrol samples were added In llle lanies 10, 11 2nd 12 ptlS717,SBTI plasmid samples
were positwe controls in the latles 13, 14, 15 atid 16. Lane 17 \+as -DNA and i, DNA-
BslE I1 marker was added in lane 18. B. PCR allalys~s of pla~~ts trat~sformed wit11
pHS723:Bt vector. Lane I
rlirougli lane I I wcrc addcd with putative twnsforn~ants
showing amplificat~on of 908 bp fragment. Lane 12, 13, 14 arid 15 had the negative
control. Lanes 16, 17 and 18 were positlve controls of pHS723:Bt plasmid vector. Lane
19 was -DNA and lane 20 was added wttll h DNA-U5tE I1 ~ilarker.