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;I! 37 'C for 3-24 Iiours 111 d;irL. Tlie iias,iy 1111~t111.c \\.;I\
rc~ii~\ed ;i~id tlie ~ I~SUC \\;IS
cleared
otT chloropli)Il by accji~c~lt~:il cli;~~~ge~ III 70-Ili(i"~ ctl1;111ol LII~II~ llsallz Iiod 110 cliloropliyll.
Allcnioli\eIy, for ditticillt tu clear tissuc ;~tid 75"" I;ictic i~cld \vnr iltidcd .ind ,111tocl;i\cd tbr 15
mlllutcs. This gl\c> ;I \cry good 111idge Cur ~)IiuIugr~~~Iiy Tlie tia5~1~ \\'I> IIIULIII~C~ III gIyce1.01
illid ob\er\ed ~lnJer a illlcroacope
~rail.dcrreti Ilno 30 1111 ILI~S
2 I5 1111 ot'e\~rac!~o~i t~i~l'lbr :~iicI I ,0 1111 oS20!o SUS \vcrz ;i~l~Ic~l, hli,~kc~i n~cll for IU scc
;i11d lilt lubes werc i~icub~tcil a1 05 degreca Ibr I0 111111
3 5.0 1111 5hl I'OI~I\~ILII~~ ~~CCIJI~ \L;I~ ;~ddcd. ~ ur~c~etl :III~ IIICLI~~I~C~ :I( U degrees Ibr 2U
llllll
1. hlo\t Iprutclllh il~i~l lpolyiic~Ili~ride> ~CIIIU\C~ iiltli llie IIIIOI~IIJIC li-tluilecyl ,ultiitc ppl.
5 Tlic snnlplc \\:15 ce~lt~~~i'~~gctl ;it 25000g ibr 20 111111 ;III~ lilte~cd lllru Miril cIol11 illlo 30
nll tube contalllilig 10 ~ nl ~supropn~iol, ~ll~xcd aiid IIICII~~IICLI ill -20Y(' for 30 111111.
0. U\A \\;la precip~t;~led '11 2OUUiJy Sur I5 111111 illid ~IIVCI.III~@ tlie lubes 011 piipcr towels
tbr 10 111111 dried pellct.
7 Tllr pcllct was rcauspended ill 5UU ~IL 'I'L 8 111id IU ILL of' IKU;lse A solution was
added (5 mdnil).
8. The samplc w;ls i~icubated n\ 37'C for 3U 111111. I vu1 (500 jiL) PI~uIIoI: ~hlorohr~n
nllxture \\as added, lii~xcd \~goruuhl) ;III~ cc~ilr~S~~gcd ;it 12,000 I~II~I /br 2 mill. Tliu
7s