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the ernblyos into plantlets on MS basal rnediun~. (Sreenivas et al , 1998). Distal ends of
cotyledons were used to induce somatic embryogenesis in Cajanus cujan by applying
BAP, kinetin and adeilitie sulfate atid whole plants were regenerated (Patel et al., 1994).
f
Direct somatic embryogerlesls was induced from excised seedling leaf segments of
/
vegetable legume, Psophocnrp~is reirngo~iolobiis by using NAA atid BAP and the
coliversioll frequency of cotyledonary embryos was 53.3% upon culture on MS medium
cor;taiili~lg ADA for 7 days followed by transfer to MS medlum supplemented with 1BA
and BAP. (Dutta Gupta et al., 1997).
2.2.1.3 Other methods
Besides orgal~ogellesis and sotllatic enlbryogenesis the techlliqucs such as
regeneration of platits from isolated protoplasts, microspore, anther and ovule cultures etc.
are less frequetltly applied in the tissue culture studies. Excellent ylelds and quality were
achieved for soyabean (Giyci~ie ~llax) protoplasts and l~latits regenerated from agitation-
derived protoplast preparations had a higher cllance of being derived from intact cells.
(Zaghnlout et al., 1990). I1rotopl;~st isolation in Araciiis iiypogu~a is relatively rare
phenomenon and there is one repor1 for isolatioll and regeneration of plantlets through this
method and tiis method was effectively used Ibr elcctroporatioll mediated tralisforniatioll
(Li et al., 1995). A different method us111g 1hi11 cell layer tech~i~que and transverse thin cell
layer (iTCL), where the ffCLs were cultured on TDZ for Piiaseolus vulguris was
employed. Shoot multiplication was enhanced uslng BAP with silver nitrate (Cawallio et
al., 2000). A reproducible protocol for plant regeneration from seedling hypocotyls
protoplasts using varlous growth regulators such as zeatin riboside, GA, and 1Bh was
reported in Vigr~a rubloba~a (Bhadra et al., 1994). Callus regeneration was achieved fro111