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2. The nie~iib~;~~ir n;ir c.~~eli~ily ~nscrtctl 111 tlic Ii~hridi/;~lio~i tube ;III~ llic pre-llyb bufkr
carefully ;~ddsd Tlic ~ ~~~-I~!~~I~IL~I~IUII
\\,15 tIo11c ~LII ,111t1111 I IICILI~ t~t 0UL'C,
3. Whlli. rlic prc-hybr~iil/;~t~w is 111 prugrcss. ~lic ~irobs \\*I\ builcd ~ br tibout IO ~iii~i. :It
100°C [or dc~~;irurnt~oii It \\as Lcp~ 111 lee ~~i~~ncd~;ircly. I'ollo\\ III~ re.~ctio~i C~II~S~IILIL'II~S
\\ere ;idded to t11c probe.
rcp:~rctl I~cslily L~IIII 2 111 c,ruhh.li~ikcr \VIIII 8 111
i\atcr suppl~sd b) tiis colilp;~~~! 1.
4 Tlic ~C:ICIIOII II~I\III~C i\:lr IIICIII?.I~~~I ,it 3jllC iur 3U 111111. ,111d 11 M:I~ ;l(I(led 10 ~IIC
liyl)r~di/;~t~o~i Ix~ttlc,
5 Tile liybrldl/;~t~(i~~ \\;I> dolic o~cr~iiglit
6 The ~nc~nbl.;ilic \\;I> lal,e11 o~it o~i tlic Ibllosring niornllig ,111tl\rab \v:~slicd w~tli ~)rilil;~i.y
iv;~sli b11t'ii.r (1;~ biii'f'cr colilpo>itio~i. rcc Appcndlu) twlcc krr I0 111i1l. C;ICI
;III~ \villi
sccu~itl,lry ua4i bull21 (fur bullbr cunipo,iliu~i, see ;'\~>~~IIL~IAJ luicc li~r 5 nii~i. c:lcll.
7 Tllc blol \v;ih trcatsd \LIIII CII)IJ-SI;I~I'I for ;ibo~t llircc I~~IIIII~C\ ;i~id\\;I\ [~rlcketl ill llie
d,:\ e1o1111ic1it C;I>SCII'.
8. AII X-ray fill11 wni kepi oil tile blot :III~ CXIIUSII~C ivi~, ~UIIC ;IS I~CI. ~IIC SI~II~I~ recorded.
9. The X-ra) film \vas \v;~sIistl ill tile folluw~~ig o~tler.
;I. Developer for ? 1ri111
b. V4;lti.r ior 30 scc.
c. Fixer for 2 tii~n.
d. Wiltcr for 2- 3 mln.
10. The developed X-r;ly filni \\'as dricd and v~cwed on the sl~de-viewer