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dose (LD-50). This cxperimel~t \bas done by ciiltilri~~g AM? expl:~nts on MS wit11 kanaolycin
(5, 10, 15, 20,25 and 30 111glL) iiiid with viiryillg COIIC~IIIMI~OIIY ofTDZ (0. 2. 4. 10 pM) 6 to
8 explants xerc cultured per plat
3.4.1 Tra~~sfornintio~~ by biolistics nlvtl~otl:
C:I~I~FIC
111:1s111id ~sol;it~t>~~ i111d I~LI~I~~C~III~II 13 IIIC Ii13t '11~1) LI I>IO~I~II~'I
111vt11od of
tt.ansforil1a1io11 iblloi\etl b) CU;IIIII~ oi' IC pl:~s~ii~d IJI~IU tllc I~I~C~UC;II.~ICI.'; ;III~ ~III~I~,I~~IIICIII
uf tile c.xpIo111s wit11 tl~c n~icruc~~s~icrs. DOIII~;I~~II~CIII C\CI~I\
\\c~c I)CI~~IIIIIC~
by I~IuI(:I~
1000/llc PIIS systcln (IjioI~~s~)il~~dcd III IUO 111. (; 1'1:
EITrA) bi~l'lkr ;111d\\ah I,cpl VII lee l'or 5 111i1i
(Cjlucow-Tris-
3 200 ILL lyals b~11'ii.r \i.,ls iitldctl: ii~bc \v;~s illvcrtcd .;c\cr,~l IIIIIC> to IIIIX IC CUI~ICII~S
lcli for 5 nlin on Ice.
3. 150 ~ IL 5 31 polu,s1~1111 ~cciolc u;~i otl~lcd, \crrlc\ccI ant1 lcli UII I ~ lilr C 5 111111.
5. The seaelion m ~xt~~re cenrrifilped Ibr ilhuut 5 111111 at 14000 rpnl ~III~ the supernata~ll
wo, traiisli.~red to n ft.csli tube (care \$as 1;1kc11 nut to c;lsry okcr tlic prccipilate or lloatl~lg
ll~,llcrlal).