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Agrobacleriuiii and ill otlier experiment transgenic platits were obtained by eloctroporating
protoplasts (Kuchuk et a]., 1990). A. lui?teJ'ucieils med~ated transformation was performed
with some members of a population of Medicogo sii/ivu into wli~ch a trait of somatic
elnbtyogenes~s was ~ncotporated vla breeding and the transge~iic pla~its were analyzed by
PCR (Du el al., 1994). A. i~oiie/i:cieilr mrdia~ed tra~isformetian of Liipiiitrs riiuiubilis was
done using shoot apical explants. A first report in tliis liarticular platit, tratisforman~s werc
contirlned with 11oi1-'idionctive Soutlicri~ iiybi.~dtfiition (U;iboogln et al., 2000) AII clllc
accession, ClAT 184, of an important paslure legume, Sly/osutilh~'~ guiuiie~is, was
transformed by Agrubac./eriiiiii-111ediated oictl~od with binary vector harboring itpill and
11idA genes (Sarria et al., 1994). A rapid and reproducible protocol for l'orfoliirr~i
subterrar~eiiin, a subterranean clover, was standardized uslng itpill, uidA and an alplia-
amylase i~iliibitor gene. Tlie protocol shows that glucose and acctosyringo~~e was required
in thc co-cult~vation medium. Four cointiiercial cultivars were successfully traiisfomed
(Khan et al., 1994). Genetic tral~sfortnation of tllc broad bean, Vicici jhbu, was done using
.4, tu~?iejircie~is and A, rhizogencs. Ttiree cultivars and mutants wcre used for the
transforniai~on stud~es (Jelenic et a]., 2000).
,,&lisiics:
Biolistic trnnsibrniation of Gl),ciiie iiiclx was done using a bovine milk
protein, p-casein cloned under seed specitic lectin promoter (hlaughan et al., 1999). The
method of electroporatlon was used to transform the protoplasts of Glycbie urgvreu.
Protoplast colonies developed into callus and 78% develop into transformed shoots that
showed nplII activity (Jones and Davey, 1991). Elnbtyogenic callus tissue from various
cultivars of Aracliis liypogaeu was used for bioiistic-mediated transformation. Callus from
mature seeds, escape free selection on liygron~ycln, brief osmotic desiccation followed by