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4.0 RESULTS
$loaf of tllc c\l~cr~~llc~lls UII ~UIII;I~IC snlbryogc~ic>~s ncre cio~~c II~III~ llic c111bryo
;I\)> cupl;l~it S~o~~ti;irtI~/;~riul~ oI' .11111ro1)11:1ti. IIIC~I~III~ 1i.r IIIC~IICIIUII 01' CIII~I.YOS \c:I~ ~UIIC
LI~III~ 2,4,5-T ;III~ 2.4-1) ;I\ lpr111ci1>;1I 1pl,111l gru\\ll~ IC~III;II~I~. I:I$ 4.1 ~IIU\VS Ille i~id~~ctii~~i
oI'c11111r)us I'ru111 III~IIIII~ c111h1)u ,1ii5 c~p1~11il rill 11511ig 2.4.5-T '15 I~IIIII;II~ $~o\\lli regulator
and 1:1g 3.2 clio\vr llic ~ndi~ct~tr~i ol' enibr)u* 11s11ig 2.-1-1). Add~l~il~i~~l ~~OLCIII reg~~li~tors
s~lch ;is TDZ. DAI'. /t.,~titi.:III~ ki1icI111 \I.CI.C 01>0 119cd 111 co~iibi~i;it~i~~i UII~ llie 2,4.5-T illid
2,4-D. ?,4,5-T co~~la~~i~ig ~iiudi;~ (JEM ssrics) T:~ble 4.1 sliowa Ihc cfict of 2 and 5 pM
?,4,5-'1' in co~iibrii,~t~on wltli TDZ, MI', ~III~IIII ur /c,~ti~l uli su~ii;it~c~iibryu ~nductioii.
Comb~~l;n~una colitainllig TDL ,111d UAI' sllowc~l \cr) luw liciji~c~icy ul'c~iibryoye~icais 111
terms of the nu~iibcr oI'rxpl;inrs respo~ldl~ig ;111d average ~ii~nlbcr oi'c~iibryoa per explant.
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The expltinta on tliese ~iied~a
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con~binat~olis prodi~ccd awdl yrcc~i niass of callus on which
two to four embryo$ ;ippt,lrcd Tlic cnibryos ;~ppc:ircd ;IS I;lrge ylubular entiricb and lliey
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did not look l~ke well-dcli~lcd c~~~bryu, III;II ;~pl~e;lrcd ill ci~li~lre ~iicd~a co~l~a~~ii~ig 2,4.5-T