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egenerated via somatic eliibryogenesis of T. rubens (Parrot and Collins, 1983). Direct
somatic embryogenesis and plant regeneration was obtained from protoplasts of red clover,
Trifoliitm pruretrse (Radionenko et al., 1994). Plants were regenerated by the direct sonlatic
embryogenesis from the cultured embryos of genus Trijoliu~i~ (Repkova, 1991). High
kequency somalic enibryogenes~s and plant regeneration was achieved from the callus
cultures of Asrrugulus udsurge~ls (Luo el al.. 1999). A protocol for regeneration vla
somatic erlibryogenesis pathway in the pasture legume Cliloria iertiuieri was developed.
Manipulation of kinerill in combi11at~o11 with IAA was found to be usethl (Dhanalaskhnl~
and Lakslimanan, 1992). Methyl jasmonate and Abscisic acid were used generally for the
maturation of induced soniatic embryos. However, their application in the soniatic embryo
induction medium was tested and they were found to be inllibitoly to tlie embryo
induction. Somat~c enibryo induction in Metliccigo sarrvu was also fol111d to be Inhibited by
ami~ioetlioxyvi~iylglycine, amino-oxyacetic
acid, 2,4-dintrophenoi and salicylic acid
(Meijer and Brown, 1988). With a view of obtaining plailts free lion1 neuroloxin, a qulck
and efricielit system was developed for r.egeticrating plants by using explents from wide
range of tissues of Lailyrur sciiiviis. An embryo rcscue method was also described to
fiicil;tate inter-spec~tic hybridizations (Misra et al., 1994). Immature cotyledons of Vignu
sinensir gave rise to somatic embryos on 2,4-D and BAP-containing medium, which
eventually developed into whole plants (Li et al., 1995). Treatment with 2,4-D followed by
BAP treat~llclil reger~ct;itcd whule plants vla ;III exuberant sonlatic embryogenesis from
leaf sections of h.letliccigo ~ujjrrtricosn (LI and Denlarly, 1996).