Download (26Mb) - OAR@ICRISAT

More documents

Recommendations

Info

Chu, P.W.G., Andersoli. B.J., Khan. M.R.I.. Sliukla, D., Higglils. T.J.V., (1999) Produclio~i ol' bra11 yellow mosn~c virus resistuot subtcrr~lieali clover (7i.,joliur1i slrb/err(rrtrlori) plaiits by tr;1nst'ormatlon will1 tllc virus co;~t protell1 gclic, ,l~i~rtils o/ App11etI Broluy)', 135: -169-480, Cuckllig. E.C.. (1060) A iiietllod for tlic ~solatlon ui' pl;llit ~)rutupli~sts ;iiid Y;ISLIUIUS. :VNIIO~L,. 187: 962-763. Culllns, W M'.. Qu;~lsct, C.0.. (1999) Urodiir'~:\rr, 111 .4grr)c~~u.~y.sr.r~1r1.s, C'I(('p,u.\,\, Hl~(,ti I(cirori, pp. 334. Cook, K.J., Ur~~ckliiirt. \V.L.. COIIISOII, J.l/ii~igiorr, D. C. Crameri. C L.. Weisseiiborii. D L., 01sli1, K K.. tirab,lu, E A., Hennet, S., I'once, E.. Grabowski, G.A., Radin, D.N., (1996) B~oproductiu~l of hunlan enzymcs ill transgenic tobacco. Anrrals of h'ew Yurk Acudertiy oJ .Tcie~rcr, 792: 62-71. Cr~ckmore, N., Bone, E.J., Williams, J.A., Ellat., D.J., (1995) Contributioii ol' the individual cornpolicntr of the 6 - endolox~n crystal to the inosqu~toc~dal act~vity of
Bucrllirs rhrrrmgre~iesis subsp. Isrcrelouis. FEMS Alicrobiology Lerrers, 131 : 249- 254. Dur, S.M., Gill, S.S., (1993) In wro arid in vivo pro~culysis of 111s Hucillrrs tirirri~rgruirsis subsp. rsioelr~rs~s CrylVD protcirl by Crrler q~ri~rclrrejas~~~uritr la1-v;rl midgut prute;lscs. 11r~eci Uiucl~e~~ris/~:~~ cr~itl hlolecrrirrr Hiologv. 23: 273-283. Dam~a~l~, F.. yen^. E., Pnolocci, F., Arc~olli, S., (1993) I~ltroduct~ori of Ihyproniyc~n reslstallce In Lor~rs spp. tlirougli /Igrohric/enir~rr ~II~~O~L'IIPA tra~~b~i)rn~:~tiw. li.rr~rsgt~~iic l~escrrrc~lr, 2: 330-335. Das, D.K , I'rnk:~sli, N.S , Blinlla S;~rin, N., (1998) An eflicrent ~cgcneratio~i systcill ol black graiii (YI~IIU II!UII~U L.) througli orga~iugcncsis, I'l~r~rr Sr.ie~rc?, 133: 109206. Datra, S.K., Petrrlians, A,, D;ltlu, K., I'otrykus, I., (1990) Gcnetic;~lly cng~~lccrcd lirt~lc ~nd~ca-rice recovered lio111 protoplasts. Uiu~/i~cl~~ioiu~~~ 8: 010-029 L);~\ey, bl,l
Page 1:
GENETIC TRANSFORMATION OF Cicer ari
Page 4 and 5:
Certificate Ger/$ed /La/ /Ae eldire
Page 6 and 7:
I deem il as a great pleastire to e
Page 8 and 9:
CONTENTS ABBREVIAI'IONS SUMMARY LIS
Page 10 and 11:
3.2.2.3 Shoot elongat~on 3.2.2.4 Ro
Page 12 and 13:
ABBREVIATIONS 2-il' 2-isopci~~cnpl
Page 14 and 15:
frequency was observed wit11 2,4,5-
Page 16 and 17:
in vitro grown plants w;is ocli~eve
Page 18 and 19:
LIST OF TABLES Table 3.1 Pr~~iier c
Page 20 and 21:
Table 4.1 1 Effect ul'age uf lhe ex
Page 22 and 23:
Figure 4.1 A - D inducl~on of sonla
Page 24 and 25:
Figure 4.9 A - C Alternative llleti
Page 26 and 27:
Figure 4.15 A, B Restriction analys
Page 28 and 29:
Figure 4.20 A, B DNA profile of gen
Page 30 and 31:
1'1.0 INTRODUCTION L~fe is the ter~
Page 32 and 33:
them. About 10% of the Earth's land
Page 34 and 35:
observations GkI Shull's experiment
Page 36 and 37:
(Nene and Haware, 1980). The legume
Page 38 and 39:
jf.0 REVIEW OF LITERATURE Large par
Page 40 and 41:
plants from them enlhuscd scientist
Page 42 and 43:
introduction into protoplasts using
Page 44 and 45:
evident by 1983 that the Agrobacter
Page 46 and 47:
The above promoters were found to b
Page 48 and 49:
gained an illstant popi~larity owi~
Page 50 and 51:
legume crop species. Cotyledon expl
Page 52 and 53:
1994). Clonal propagation of FI int
Page 54 and 55:
were induced to for111 callus when
Page 56 and 57:
' the ernblyos into plantlets on MS
Page 58 and 59:
and nprII for selection. PCR and So
Page 60 and 61:
Agrobacleriuiii and ill otlier expe
Page 62 and 63:
ioteclinologicsl i~iiprovemel~t of
Page 64 and 65:
combinat~ons and concentrdtions of
Page 66 and 67:
egenerated via somatic eliibryogene
Page 68 and 69:
egenerat~on from protoplasts and ca
Page 70 and 71:
transformants were identified by sy
Page 72 and 73:
urgent econoniical control measures
Page 74 and 75:
applications of traps. Technically
Page 76 and 77:
in the laboratory and releasing tl~
Page 78 and 79:
have been exhaustively reviewd (Dai
Page 80 and 81:
larvae of certaln Insects were unab
Page 82 and 83:
3.1 Pl:~nt 111nteri:il sllrl cultur
Page 84 and 85:
0.5. 2.0 and 5.0 pM were added. 111
Page 86 and 87:
Lec!/i?is: Young anci juve~iile lea
Page 88 and 89:
AM3 explant: This was a by-product
Page 90 and 91:
PM) 2nd GAJ (2 5 PM), eltller singl
Page 92 and 93:
of the plastic bag was removed rwlc
Page 94 and 95:
- . -- laopropyl alcoliol ; Absolul
Page 96 and 97:
The sl~des were Illell muiaited and
Page 98 and 99:
6. The bupcniatant \\,I,, t,ikcli i
Page 100 and 101:
5. Microcal~lers were allowed to se
Page 102 and 103:
till the growtli of bacterial cells
Page 104 and 105:
;I! 37 'C for 3-24 Iiours 111 d;irL
Page 106 and 107:
PCN for 111cl.4 ge!iiJ: I'C'I< tbr
Page 108 and 109: 4. Tliu pel \\as \r;~alicd 3 ti~iir
Page 110 and 111: 4.0 RESULTS $loaf of tllc c\l~cr~~l
Page 112 and 113: These combi~iatiori sl~owed liiorc
Page 114 and 115: egeneratloll \!;IS dli'licull ;~nti
Page 116 and 117: decreased a[ cllkalinc pH \+li~le 1
Page 118 and 119: either side of'bws;tl portiol~ oill
Page 120 and 121: IBA sl~owed bro\\~~i~~g i~lld de:~l
Page 122 and 123: humidity uf 50% 111 the dayrinie a1
Page 124 and 125: 4.2 Histological studies ~II nlultl
Page 126 and 127: sliowetl no liirtlie~ yrowlli Tllc
Page 128 and 129: Conlirmntioll of plllati!e t~.~ll~a
Page 131 and 132: 5.0 DISCUSSION During tile la31 dcc
Page 133 and 134: leatlets (Dilieshkuniar er al.. 109
Page 136 and 137: used with an add111ou;ll care :u~d
Page 138 and 139: tissue to regenerate illto lesser i
Page 140 and 141: prololigcd culture on TUL sho\\~d 1
Page 142 and 143: exceed 5 cni Icngtl~. Loiigcr shoot
Page 144 and 145: stem and lravca Secol~d;lrq ~'IIICI
Page 146 and 147: arnplitication of the sopccIi\i. 90
Page 148 and 149: Explant Preparation -Geminate seeds
Page 150 and 151: 6.0 REFERENCES Adkins, A.L.. Godwin
Page 152 and 153: Avenido, R.A., Hattori, I
Page 154 and 155: Benfey, P.N., Chua, N.1-I., Ilcgiii
Page 156 and 157: Cnrvalho. IC1.H C dc , Uui Vat1 LC
Page 160 and 161: Deblaere, R., Reyneerts, A., Hotte,
Page 162 and 163: Drun~mond, M.H., Gordoil. M.P., Nes
Page 164 and 165: Foster, J.G., Coulombe, B.A., Van S
Page 166 and 167: Gill, R., Saxstla, I'.K., (1992) Di
Page 168 and 169: Hess, D., (1970) Versueche zur tran
Page 170 and 171: Hopkinson, M.J. ;md Collins, I1.M.,
Page 172 and 173: Karthikeyan, A.S., Sarina, K.S., Ve
Page 174 and 175: Krens, F.A., Molendijk, L., L., Wul
Page 176 and 177: Li, Z.Y., Tanner, G.J., Larkin. P.J
Page 178 and 179: Maxan~, A.M., Gilbert, W., (1977) A
Page 180 and 181: field conditions. Procc.rdbigs o/Na
Page 182 and 183: Ohara, A., Akasako, Y., Daimon, H.,
Page 184 and 185: llechrnlics sur les prnidilies des
Page 186 and 187: Rand, G.M. (Ed.) (1995) Ftordir~~rc
Page 188 and 189: Richardson, M.J., (199 I ) Seed sto
Page 190 and 191: Sarria, R., Calderon, A., Thro, A.M
Page 192 and 193: Shri, P.V., Davls, T.M., (1992) Zen
Page 194 and 195: Tegeder, M. Koli~i, I-I., Nibbbe, M
Page 196 and 197: Venkatachalam, P., Klshor, P.B.K.,
Page 198 and 199: Winans, S.C., (1992) Tiyo way chemi
Page 200 and 201: 1 APPENDIX 1. hIURASRICE AND SI(SO)
Page 203 and 204: Table 4.1 Induction of sorrlalic er
Page 205 and 206: Table 4.3 111ducti011 of en~bryos O
Page 208 and 209:
'Table 4.6 Dificrent combinations o
Page 210 and 211:
Table 4.8 Effect oI'TDZ, 2-iP :~nd
Page 212 and 213:
Table 4.10 Effect of i~lclusio~~ or
Page 214 and 215:
Table 4.12 Induction of multiple sh
Page 216 and 217:
Table 4.14 Effect of media constitu
Page 218:
Figure 3.1 Dii~gralll~l~i~tic rcpre
Page 222:
Figure 3.3 A - C Diagrn~nn~atic rep
Page 225 and 226:
I . Nhu 1 51 ?C,%- .pal5125 &pa1512
Page 228:
I
Page 232:
Induction of somatic embryos from m
Page 238:
I.iy111. J.5 SI:i;i.\ iil ~ ~ l ~ ~
Page 254:
IIistologic:~l stutlic\ of dcveiopr
Page 258:
Rc\[ricfiot~ ;111;1l)si\ ur ill? ~
Page 262:
I'C'R :~llipLiiir:iriuil 01'700 bl)
Page 266:
Fixi1r.c 17 \r:15 -l)K,.\ ;!lid J"
Page 272:
1:igurr 4.22 Sui~lllcrn :III:II!s~$
show all

Download (26Mb) - OAR@ICRISAT

Create successful ePaper yourself

Delete template?

Save as template?