Download (26Mb) - OAR@ICRISAT

gained an illstant popi~larity owi~ig to 11s cfticic~icy and localiza~ion of the exprcssio~l
without extrnctiiig llie tissue. The luoiferase reporter gelie bas developed by de Wet a1111
associates (De Wet el al., 1985) and was reviewed by Luelirsrn el al. (1992). The
gene
that codes for green fluorescelit prote~~i (GFP) *as the recellt one isolated from jcllytish by
Chalfie et al. (1994). Many reports apprarcd In support snd against tlie usage of GFP
(Ilaseloffai~d Amos, 1995).
f
2.2 Legume tissue culture and tra~~sfor~iiatio~~
Leguminosae is a very i~i~portant falllily of angiospcrnls comprising of ~nany
species in relation to Iiuman nutr~tion, pasture and tbdder needs. Important protein rich
seed bear~ng plants, n~ostly lierbaceous, such as pros, lentils, beans collectively known ss
pulses are members of this filmily, They rank next to cereals in tenns of human nutrition.
In qua~ititative significance they are far bchind the cereals, however, gaining some due
inlportancc as food additives ill tlie recent yc,irs. Domi~~ot~ol~ of tlic cereals in tlie food
sector allowed oi~ly miirpinal ~~icreascs in tile overall yield of pulses. I