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The sl~des were Illell muiaited and b'icued ulldcr ~ni~croacclpc. The nuclei stained
bluish violet and cytoplasm in ranous sh;ides of piilk.
Ge~ietlc transfiir~~i;ii~o~i of rllc AM4 c\p1;1111> \v,i:,
dtil~c \in b~ul~a~ic :I> \i'cll :IS
.~~~O~~IC/~I~~~IJII-III~~I;II~~
111et11otls. BI~II~IIC IKII~\~U~III;ILIOII \\;I> LIOIIC liy LI\III~ 111c ~>l~~s~ii~d
pllTL)Y:(;US-ll~t (6.7 ib) (i:lp 3 3) (\'cclor \\,IS Lllltlly jpiu\idcd b) Dr I 111!tll gellc .is aclccr:ible ~~~orker, IIII/I\ gc11c \\ 1111 ;it1 ~lllcr~~lcrsild 1111ro11 (GUS-llll)
;is the reporter allel ;IIII~)~CI~~II~ IC~I~~CIIICC pe11e I~II. OIi~tcr~~~I >CICCIIOII. 11~11111/1/11 i111e1 iiidA
genes \\ere under lllc rcgiilat~o~~ ul'C;1I\1V-355 proiliotcr. I\ \;illel) (11'
\111glc ;lntI n~t~lt~plc
cluiiil~g altes wcrc prcaclit ;it diili.relit luc;~~ioiis 011 tlic pl,i>lnid 'Tlic ; ~,~Iu~~I~I~~~IIIII
nletllod ir,ls ~>crhri~ied by 11ai11g t\io iiii~,ir) \~CIOII,
ii;i~i~cIj, pI1S 723:131 (I,ig 3.4) and pIIS
737:SUTl (Flp 3.5) (bull1 the \cc~ora\vcl.c kl~itily ~)rov~clcil hy 111.
(; Scli;lr;~j, I'I;IIII
131otecl111ology li~~l~~utc.
Siiaka~uon. C'ii~~id,iJ \~'IIICII II;II~OIC~ U/('~:~l:ll) ;tiid SU77 ;la
;~grono~~~~c;illy inlpurtal~t genes real~e~tivcly. Uutl~ rlic vscton 1i;lic 111iiA r ~ 11/1/11 ~ ~ gc11es d
ll~sed Intu ailigle UIIII
tlrlveli by C'aMV 35s prolilotcr. Ui0.1.l:lh gcnc \v;li ilr~vei~ by doublc
35s proniotcr where ;la SUl7 gciic ir;ia dr~vcii by \~iiglc 35s Iproilll~c;~lio~~ t11;il C~II
operate both III E. (oii as i\cill aa ilgrubot/ei.~ii~i~ ;III~ otl1e1. at111b~1tcs aucI1 ;IS ~~i~~ltiple C~OII~II~
SllCS.
In borl~ biolistic ;is \$ell as dyrohot /eri~i~ii-~?lciIinlcd procesw procedi~res tl~e putolive
transforn~iints iverz obt;i~~~cil b) ~elccling llle t~-;iiirliir~i~n~n, using 111~111 as tile sclcctlng gcnc
and kananiycin as tlie ant~b~otic I'or iclcctioli. Control uxplanb wurc used lo test the lethal