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from germinated seeds of a multipurpose legulnino~is tree. Srsboi~in graildifolia. showcd u
high percentage (96%) of expli~nts prod~icilig .n least 30 slioot bud per expinlit (Iletre~ c~
al., ~YY~J.
Irii111~~c.l o~~flriogcvw~i~: Regcncri~tioli ~III(I ~IIJI~SIS ur c;~llus li.olii Ilu\v-sorted
heterokaryolls of soybeall (Gli,c,~ric 1rici.1) mid 6' c.ciir~,sc,c.irs was donc (Haliiluatt ct a1 .
I9S8). I'lcces
of 1e:rvcs li-o~n sectll~ligs ot' ,liii~,/ii\ jirii/oi ivclc IC~L'IICI;IIC~ \I;I
orgaliogelieals a~ld so~li;~tlc e~iibryogcncsis path\\.;lys. Plant rcgelleratioli was obtaillrd
I;I
two developmentnl path\vays o,-ganogenesls and soniatic eniblyogenesis. Orgaliogcnic
callus cultures were i~illiatcd Sroni pleces of leaf oli MS niediunl suppleliirlitcd wltli NAA
or 2,1-D ill culiiblliatiuli \villi BAP, kinetill or 2-IP. Tlie niust sui~able co~~ibi~iat~u~i for
plant rege~ieratioii tliruugl~ orgn~iuge~icsis was a11 iliitial lliediuli~ cornposed ol' NAA and
BAP fbliowed by transfer ot'the callus to a slioot inductioli medium (MS+ BAP) Routing
of regenerated shoots was read~ly acli~cved by culture on MS w~tli NAA. Embryogenic
callus cultures were inltialcd froni plcces of leaf on MS n~ediurn supplenlented with
picloram ~n coliib~lietio~l w~ih kitletin, Aeatlil, BAP or 2-iP, alid the iiioat suitable
comblnat~ons wcre picloram, BAP or 2-il'.
(Iky ct al., 2000j. A comparative study of
callus t"ormation and pl;~nt regelicratlon was dolie using d~fttrciit explants of Phuseolus
vulgaris and P. coccitieus (Ruiz et al., 1986). A different sylithctic auxin 2,3,5-
trilodobenzoic acid was sllowii to ~nduce callus atid roots on stem cuttings of niungbeali,
Phaseul~is aureus (Ali and Jarvis, 1988). A novel liietliotl of culturing lcaf disc explnnts of
pigeon pea (Cojrirruv ccijtrri) on ~~iulril)lc slioot ~liduction niediuni with IAA and BAP where
the shoots originated via callus phase (Itathore ct al., 2000). Plants were regenerated fro111
leaflet-derlved callus of Aescl~)~noir~oie setisiliva, A. urrie~icunu and A, villosu. Explants