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transformants were identified by sylitllesis of opines in the shoots. An important fodder
legume, Astrogalus sinicus was transformed by A rhizogrr~es atid the uidA gene activity
was confimied (Cho et al., 1998). The regenerants exhibited Ri-plasln~d syndron~e (sn~all
thin lcaves and short internodes) arid 55% of tlle seedlit~gs diowed GUS activity and
positlve gene integrations. Genetic transforniation of tile pasture legurne and the model
plant for study~ng Rllizobilrn~ legume symbiosis, Medicugo truncorulu was reliably
transforti~ed wtth b~nary vector harboring ~iptll and rriclA and NOS atid CaMV 35s
pronioters respectively. The TZ geileration of the platits showed Mendelian inheritance of
the integrated transgenes (Wang et al., 1996). A rapid regeneration system from
cotyledotiary node explants was used for stabbing and injection co-cultivation of
Agroboclcri~r~rr, In Pisu~ir sutivunr (Jordan and Hobbs, 1993). Genetic transibnnatron via
somatic embryogenesis pathway with immature leaflets and l~odal explants of pasture
legume Lutlryr~rs sarivus was reported by Barlia and Mehta (1995). Mature de-
enibryonated cotyledons with intact proximal end of Yigrto rorgrritulutu were used for
Agrobacreriu~?~ transfortnation. Over 15% of the shoots were selected on selecttoti
medium.
Iritegration of Itpt gene was detected by usi~ig Southerti blot analysis
(Muthukumar el a]., 1996). Ri plasmid atid dlszir~iied 1 i plasmids were used to transfonn
Mediccigo trnnccrttrlo. Genes of Ri plas~iild negatively ~nterf'ercd with
soinatlc
embryogenesis. Only Ri plasmid with an inactivated rol A gene regenerated transgenic
plants (Thomas et a]., 1992). Loitis jupoilicris is a ~iiodel plant for Rhirobium host
interactions. A. rhizogenes mediated transformation was perfomied and hairy root
formation was observed. Most virulenl strains A, rliizogenes for this species were also
found (Stiller et al., 1997). Lorrrs cor~riculatus and L. lenuis were transforn~ed wtth A.