- Page 1: GENETIC TRANSFORMATION OF Cicer ari
- Page 4 and 5: Certificate Ger/$ed /La/ /Ae eldire
- Page 6 and 7: I deem il as a great pleastire to e
- Page 8 and 9: CONTENTS ABBREVIAI'IONS SUMMARY LIS
- Page 10 and 11: 3.2.2.3 Shoot elongat~on 3.2.2.4 Ro
- Page 12 and 13: ABBREVIATIONS 2-il' 2-isopci~~cnpl
- Page 14 and 15: frequency was observed wit11 2,4,5-
- Page 16 and 17: in vitro grown plants w;is ocli~eve
- Page 20 and 21: Table 4.1 1 Effect ul'age uf lhe ex
- Page 22 and 23: Figure 4.1 A - D inducl~on of sonla
- Page 24 and 25: Figure 4.9 A - C Alternative llleti
- Page 26 and 27: Figure 4.15 A, B Restriction analys
- Page 28 and 29: Figure 4.20 A, B DNA profile of gen
- Page 30 and 31: 1'1.0 INTRODUCTION L~fe is the ter~
- Page 32 and 33: them. About 10% of the Earth's land
- Page 34 and 35: observations GkI Shull's experiment
- Page 36 and 37: (Nene and Haware, 1980). The legume
- Page 38 and 39: jf.0 REVIEW OF LITERATURE Large par
- Page 40 and 41: plants from them enlhuscd scientist
- Page 42 and 43: introduction into protoplasts using
- Page 44 and 45: evident by 1983 that the Agrobacter
- Page 46 and 47: The above promoters were found to b
- Page 48 and 49: gained an illstant popi~larity owi~
- Page 50 and 51: legume crop species. Cotyledon expl
- Page 52 and 53: 1994). Clonal propagation of FI int
- Page 54 and 55: were induced to for111 callus when
- Page 56 and 57: ' the ernblyos into plantlets on MS
- Page 58 and 59: and nprII for selection. PCR and So
- Page 60 and 61: Agrobacleriuiii and ill otlier expe
- Page 62 and 63: ioteclinologicsl i~iiprovemel~t of
- Page 64 and 65: combinat~ons and concentrdtions of
- Page 66 and 67: egenerated via somatic eliibryogene
- Page 68 and 69:
egenerat~on from protoplasts and ca
- Page 70 and 71:
transformants were identified by sy
- Page 72 and 73:
urgent econoniical control measures
- Page 74 and 75:
applications of traps. Technically
- Page 76 and 77:
in the laboratory and releasing tl~
- Page 78 and 79:
have been exhaustively reviewd (Dai
- Page 80 and 81:
larvae of certaln Insects were unab
- Page 82 and 83:
3.1 Pl:~nt 111nteri:il sllrl cultur
- Page 84 and 85:
0.5. 2.0 and 5.0 pM were added. 111
- Page 86 and 87:
Lec!/i?is: Young anci juve~iile lea
- Page 88 and 89:
AM3 explant: This was a by-product
- Page 90 and 91:
PM) 2nd GAJ (2 5 PM), eltller singl
- Page 92 and 93:
of the plastic bag was removed rwlc
- Page 94 and 95:
- . -- laopropyl alcoliol ; Absolul
- Page 96 and 97:
The sl~des were Illell muiaited and
- Page 98 and 99:
6. The bupcniatant \\,I,, t,ikcli i
- Page 100 and 101:
5. Microcal~lers were allowed to se
- Page 102 and 103:
till the growtli of bacterial cells
- Page 104 and 105:
;I! 37 'C for 3-24 Iiours 111 d;irL
- Page 106 and 107:
PCN for 111cl.4 ge!iiJ: I'C'I< tbr
- Page 108 and 109:
4. Tliu pel \\as \r;~alicd 3 ti~iir
- Page 110 and 111:
4.0 RESULTS $loaf of tllc c\l~cr~~l
- Page 112 and 113:
These combi~iatiori sl~owed liiorc
- Page 114 and 115:
egeneratloll \!;IS dli'licull ;~nti
- Page 116 and 117:
decreased a[ cllkalinc pH \+li~le 1
- Page 118 and 119:
either side of'bws;tl portiol~ oill
- Page 120 and 121:
IBA sl~owed bro\\~~i~~g i~lld de:~l
- Page 122 and 123:
humidity uf 50% 111 the dayrinie a1
- Page 124 and 125:
4.2 Histological studies ~II nlultl
- Page 126 and 127:
sliowetl no liirtlie~ yrowlli Tllc
- Page 128 and 129:
Conlirmntioll of plllati!e t~.~ll~a
- Page 131 and 132:
5.0 DISCUSSION During tile la31 dcc
- Page 133 and 134:
leatlets (Dilieshkuniar er al.. 109
- Page 136 and 137:
used with an add111ou;ll care :u~d
- Page 138 and 139:
tissue to regenerate illto lesser i
- Page 140 and 141:
prololigcd culture on TUL sho\\~d 1
- Page 142 and 143:
exceed 5 cni Icngtl~. Loiigcr shoot
- Page 144 and 145:
stem and lravca Secol~d;lrq ~'IIICI
- Page 146 and 147:
arnplitication of the sopccIi\i. 90
- Page 148 and 149:
Explant Preparation -Geminate seeds
- Page 150 and 151:
6.0 REFERENCES Adkins, A.L.. Godwin
- Page 152 and 153:
Avenido, R.A., Hattori, I
- Page 154 and 155:
Benfey, P.N., Chua, N.1-I., Ilcgiii
- Page 156 and 157:
Cnrvalho. IC1.H C dc , Uui Vat1 LC
- Page 158 and 159:
Chu, P.W.G., Andersoli. B.J., Khan.
- Page 160 and 161:
Deblaere, R., Reyneerts, A., Hotte,
- Page 162 and 163:
Drun~mond, M.H., Gordoil. M.P., Nes
- Page 164 and 165:
Foster, J.G., Coulombe, B.A., Van S
- Page 166 and 167:
Gill, R., Saxstla, I'.K., (1992) Di
- Page 168 and 169:
Hess, D., (1970) Versueche zur tran
- Page 170 and 171:
Hopkinson, M.J. ;md Collins, I1.M.,
- Page 172 and 173:
Karthikeyan, A.S., Sarina, K.S., Ve
- Page 174 and 175:
Krens, F.A., Molendijk, L., L., Wul
- Page 176 and 177:
Li, Z.Y., Tanner, G.J., Larkin. P.J
- Page 178 and 179:
Maxan~, A.M., Gilbert, W., (1977) A
- Page 180 and 181:
field conditions. Procc.rdbigs o/Na
- Page 182 and 183:
Ohara, A., Akasako, Y., Daimon, H.,
- Page 184 and 185:
llechrnlics sur les prnidilies des
- Page 186 and 187:
Rand, G.M. (Ed.) (1995) Ftordir~~rc
- Page 188 and 189:
Richardson, M.J., (199 I ) Seed sto
- Page 190 and 191:
Sarria, R., Calderon, A., Thro, A.M
- Page 192 and 193:
Shri, P.V., Davls, T.M., (1992) Zen
- Page 194 and 195:
Tegeder, M. Koli~i, I-I., Nibbbe, M
- Page 196 and 197:
Venkatachalam, P., Klshor, P.B.K.,
- Page 198 and 199:
Winans, S.C., (1992) Tiyo way chemi
- Page 200 and 201:
1 APPENDIX 1. hIURASRICE AND SI(SO)
- Page 203 and 204:
Table 4.1 Induction of sorrlalic er
- Page 205 and 206:
Table 4.3 111ducti011 of en~bryos O
- Page 208 and 209:
'Table 4.6 Dificrent combinations o
- Page 210 and 211:
Table 4.8 Effect oI'TDZ, 2-iP :~nd
- Page 212 and 213:
Table 4.10 Effect of i~lclusio~~ or
- Page 214 and 215:
Table 4.12 Induction of multiple sh
- Page 216 and 217:
Table 4.14 Effect of media constitu
- Page 218:
Figure 3.1 Dii~gralll~l~i~tic rcpre
- Page 222:
Figure 3.3 A - C Diagrn~nn~atic rep
- Page 225 and 226:
I . Nhu 1 51 ?C,%- .pal5125 &pa1512
- Page 228:
I
- Page 232:
Induction of somatic embryos from m
- Page 238:
I.iy111. J.5 SI:i;i.\ iil ~ ~ l ~ ~
- Page 254:
IIistologic:~l stutlic\ of dcveiopr
- Page 258:
Rc\[ricfiot~ ;111;1l)si\ ur ill? ~
- Page 262:
I'C'R :~llipLiiir:iriuil 01'700 bl)
- Page 266:
Fixi1r.c 17 \r:15 -l)K,.\ ;!lid J"
- Page 272:
1:igurr 4.22 Sui~lllcrn :III:II!s~$