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Protoplasts were isolated from immature co~yledons of G/yciile soja. These
protoplasts started to divide after 3 days of culture and the division freqtle~lcy of
protoplasi-der~ved cells counted at 12 days was 36.8%. Slioot buds were regencrated on tl~e
surface of the nodular celluses with a frequency of25% \\hen tlic cnlluses werc placed OII
MSB medium with IAA and BAP. Wliole plants were regencrated uiio~i transfcr of 3-4 cm
shoots to 50% MS mediu~ni witli IDA (\\lei n11d Xu, 1990). A brcnhtl~rougli In the pl,lnt
regencratlon from tile protoplasts isolated from Vicia jrrba and V. tror6oire1r~i.s. I'rotupl;i,i~
of 10 cult~vars were isolated l'rom etiolated slloot lips and tested for their regcnc~~lt~o~~
capacity. After purification, protoplasts were embedded in sodiun~ algitiatc and cultured 111
the medium contait~ing 2,4-D, NAA and BAP. Division frequencies of up to 40% werc
obtained. Six weeks afier cmbcdding, protoplast-dcrivcd calluscs were transfcrrcd to
Gelrite-aoiidified mcdin wit11 different colnbinationa of groivtli rcgulalors (Tegeder et al..
1995). Low voltage treatment and nurse cells from Medicngo ~crriva were used to
regenerate callus from protoplasts isolated from seedling aiid suspcnsio~n cultures of
7i.i/olirii11 slrb~errri~~eii~ir (Li et al., 1990). A protocol Tor the isolatiorl of root proloplasts
from Vigi~ri rridiara and leaf nlesopl~yll protoplasts of ~niotlibean, V cico~ritijolia gave a
plating efficiency of 1.3% and 2.81% respectively. Shoot mcristemoida devcloped o~lly
from mothbean, into shoots and later lrlto ivholc piants (Avenidu et al., 1993). Genotype
dependent protoplast ~regencration into wlloli: plants was observed in red clover (Trfiliioli
pruiense).
Protoplasts were derived from leaf and suspelision cultures of the culi~var
(Myers et al., 1989). Cull suspension cultures were grown and plantlets were regenerated In
Indigofera
eni~eapl~ylla (Bharal and Rashid, 1984). In vitro conditions for plant