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Figure 4.20 A, B
DNA profile of genomic DNA isolated from putative transfonnants of chickpea. A.
Purified DNA profile of genomic DNA of BlCrylAb plants. DNA was prepared in
duplicate samples. Lanes 1 to 10 shows first set and lanes 11 to 20 shows the second set.
B. Effect of amount of BSA, water and enzyme in the reaction mixture to digest the
genomic DNA sample of chickpea. Unless otlienvise stated, the Lola1 volume of reactton
mixture was maintained at 25 p1 with 10 p1 of genotnic DNA, 2 pI (I0 units) of clizpe,
2.5 pl of reaction buffer and the rest being water. In case of volume variations arising due
to tile changing enzyme and water collcenlratioils, tlie reaction buf'fcr volu~ile \\,as
maintained accordingly with its working concentralion always kept at IX.
Lane 1: Unrestricted genoliilc DNA (5 pg, was added to tile well)
Lalie 2: 1 p g genomic DNA digested with I0 ltnils of enzyme
Lane 3: 5 pg, gelionitc DNA digested willt I0 units of enzyme
Lane 4: 5 pg. getlomic DNA digested with 15 units of etizynie and dot~blc llic
quanlily of haler
Lane 5: 5 pg, ge~iolilic DNA digested \z;~lIt 20 units of eti/.ylne with double llle
quanttly ofwaler
Lanc 6. 5 ~tg ~CIIOI~IC DNA digested will1 10 utllts of elizytiie and 0.1% BSA
Lane 7: 5 pg, genornic DNA digested w~tll IO units ofcn~yme, 0.1% of BSA and
double tlie quantity ofwnler
Lane 8: 5 pg. getiomlc DNA digested \villi IO ~~iitls ofcnzynle, 0 356 of BSA
Lane 0: 5 pg, getioniic DNA digested wit11 I0 ullils of enLytne, 0.3% of USA and
double tlie cll~antity of water
Lane 10. 5 pg. gellolnlc DNA digesicd will1 IO i~rlits of cnzyme, 0.5% of BSA
Lane 11: 5 pg. genomic DNA digesled will1 10 units of enzyme, 0.5% of BSA
atid double the quantity of water
Lane 12: 5 pg, genonitc DNA digested with I0 unlts of enzyme, 1.0% of BSA
Lane 13: 5 pg, genonltc DNA digested with 20 itnits of enzyme