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ofpH on 1~111l11ple slloor rtlditcrio~r: The shoot induction nlediuni (SIM) was
prepared with pH mriations of 4.0, 4.5, 5.5, 6.0, 6.5, 7.0, 7.5 ailti 8.0 prior to autoclavi~lg,
Solidificatio~~ of the ~iiedii~~~i \\as corufully ~ilon~torrd and the prepared AM2 cxpla~lts were
culturud on [lie SIM with abo~e-nlcntio~ied pi4 v;iriatio~is.
Role of
co(vletlui~ni~' iis~~~r: Co~iiplete or parti;il inclusioil of co~yledon w~tll the
regenerating expla~lt. 011 mult~ple slioot i~ldiictiun ficcjilcncy wiis slud~ed. Tlie AM2 explallts
were prepared accord~~ig tu tlie proci.durc nisntiuncd abovc and illc cotyledon portion was
surgically exc~sed ci~lier co~iiplstcly or pa~.ti;~lly (I~~ilt) and one set ol'c~pI,iilts were cultured
witli inclusion of co~nple~u cotyledo~l. 'The rcsulti~ig cxpl;inta ivcrc ci~ltured on SIM witli a
tlclis~ty of6 to 8 sxpl;~~its per 1)1;1tc.
CUIII~)OI.II~I~~L,
IIII~/~I/I/~, ,/iooi iiiilirti~o~~ liviii i/~/]e~.r~~ii ci.i~/coio M:il;irc c111Oryo axis.
sliool lip, i~x~llary butl. i\hl I, AM?. AM3 011~1 Ah14 CAI)~;III~> \\ere P:c~~~I:c~ d~c(~rcliilg to tllc
procedul-es rncntlo~icd III tllc 5ccti{1113.2 2.1 ii~ld cu1111:cd UII Slhl. Nu111bi.r uf rcslio~iding
exl~la~lts and inunlbcr oi' I~ILIIUIIIC
before their trunslir to tlic ~lioul clu~lp:~iiun mctliii~ii
$110uts ]per C.X~~;IIII bere rcc~r~lud 111 lllc third ivcck just
Age o/ [lie \cctl/iiiy: I3as11ig UII tllc ~[>li111t I>~UI):I~;I~IUI~ sIr:~Iegy, d~lli'rc~il ehplillits
\sere derived boll1 hecdllngs of iarluu ;I~A. Ahll. Ahl!,
AM3 a~id Ah14 cnplanls were
prepared accord~~lg to tl~eirc,pectivc proccdi~rca ~ncn~ioncd abow konr 2.1. 6. X, 10, 12, 14
and I6 day-old seedlings. Tlic rcsuli~iig cxpl;ints werc culiiired o11 SlM.
3.2.2.3 Shoot elongatioll:
The hiloot elo~igatio~i 11iedi11m (SEM) bnslcully coiis~stcd ol'lo\rcrcd co~~cc~~tration of
plant growth regulators, %lie11 compared to tlie r~tdi~ction rncd~~~ln Various plant growth
regulators ~ ~sed for this pnrposc werc 2-il' (2 and 5 pM), BAP (2 i111d 5 pM), kiiiet~n (2 and 5
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