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germinating the seeds of pasture legumes, Lutlt,~~us ticrra, L, oclr~~ris and L, sativus. Best
nlultiple shoot frequency was obtained on ~ilrdiuili contain~ng 50 pM BAll (Malik et al.,
1992). Mulliple shoots were induced born slioot tip explan~s of iii vitro grown seedlings of
iWacror)~io~~~ii ii~iijluriri~i. Effects of ;tdclllne sulfatc. BAP and IBA were studied
(Varisaimoliamcd ct al., 1999). High frequency regeneratio11 of adventitious shoot buds
was observed in Pi~~ilit suti~~ir~ii. Silver nitrate did not sllow :my promotive effect on
nlultiple slioots but resulted in slloots \vttli ucll-dcvelopsd tetldrtls and large stipulcs
(Ozcan et al., 1992). Young i~lflorzscenccs of Cerutu~iic~ silic/uu were cultured 011 MS
mediuni supplenlerited with BAP with various concentrations of casein liydrolysate fbr
obtaining multiple shoots (Bl~alerao and Chinchanikar, 1992). Luthyrlis sj~lvestris (flatpea)
is an important forage legume especially in acid~c soils. Hypocotyl derived multiple shoot
systeni was developed for the clonal propagation of this species (Foster et al., 1991). All
efficient micropropagation metllod was developed for Scsbo~licr rostru~u by opt~~li~z~ng
parameters 11ke variations in the basal mcdiulli and pilotoperiod changes (Pellegrtneschi
and Tepfer, 1993). Mult~ple shoot rcgenvrurion was observed fro111 Immature seedl~ng
explants of Lupiriu~ ~riu~ubilis by using TDZ with modified Schenk and Hildebrandt
lnediulrl (Rah~m et a]., 1999). Complete plants of Lupi~rus iutetrs were regenerated from
hypocotyls segments and were eficielitly ~nodulated by Brudyrliizobiu~n sp. (Dam and
Chamber, 1993). A~~tityilis cytisoides, a legulne shrub used for aforestation and reclamatiori
was successfully regenerated fro111 both juvenlle (cotyledonary nodes and apical buds) and
mature (axillary buds) explants (Gavidia et al., 1997).
Indirect orgunoge~~esis: Plant regeneration was achieved via callus phase using
hypocotyls explants of an irnporta~it forage legume Astruguius udsurzens. The