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Lec!/i?is: Young anci juve~iile leallers, semi-iiiaturi. leatlets ti.oni thc ni~ddle portion of
the seedli~ig and ~ii;~tiire leallcts ti.otn tlie bilsal p;itls of7-day-old scedli~ig were sep;lr;~ted and
cultured oil SIM.
Lrq Dii~e.
Tlie dr-co:itcd scuds wcrc pcr~ii~~i,~tcd u~i hlS lbr 7 d.~yb 11r1or to obt;111111ig
tile leal'cxplti~it. Lcal'b;~sc (pct~olc base) II~;I ~iic~~stircd ;~but~t 3 111i1i W;I~ C\CI~C~ ;111il c~~lttircd
on tiit SIM.
//ip~~~o(~/: The orcr~i~giit so;iked ;il~ dc-co;rlcd jccds \vcrc eriii~~i;i!cd oil MS and 4
to 5-day-old seedlings were bclcclcd for dcr~v~iig tlic ~~I~;IIII 'Tlic stelii 11;11t that was just
abo\e tile cotylcdon,iry liotlc jt~~iclio~i iv~~h csc~vtl a~~tl 111s sliout 111) rugion was rcniovcd. Tlic
explant tha ~iieasurcd :iboui 4 iiim \v\.;ls cululred on SIM.
Epicu~l: Tile ove~.nigIit soohctl and dc-co;~tcd scccis \+ere pcr~iii~i:ilctI on MS and 4 to
5-day-old sccds \\ere stlccted ib~. obl;ri~iil~g tlic c\pl;~~it Tlic ~~ly)er niost Iparr ol'lhc tap root,
just below the cotyledoii:rry iiodc jt~~ictiu~i \+;I\ exc14 and tlie cxpl;i~it ~iicasuring 4 mm
devoid ol'roor 1111 rcglon was cult~~rcd on the ~~iductioti ~ncdiu~ii.
Roo, A?~I?I~,II/.Y "1111 I.UIJI /I/). Root \egliielilb ;111tl root lip MCSC ehcibcd fro111 7-day-old
sccdlingr and cultiired ~epa~.ately on tlie i~itlt~ctio~i iiicd~ii~ii.
A.~rIlory bi11i. T\io types axillary bud uxpla~ila wcrc prcparcd. Tlie ~iia~jor v:iriatioii for
preparat~on of these cxpla~its wt~s their gsl-~iii~lation paltcrli. Tlic first onu, dcsignatud aa ABI,
was prepared by excisi~ig tlie avillary bud from a 7-day-old secdli~ip hat was gerniiliated on
hlS medium. Tlils nx~llary bud ~iieoaured around I to 1.5 mm In leiigtll. Tile second one.
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