Download (26Mb) - OAR@ICRISAT

Cold shock: Tlir explants benr~ng the eli~bryos \\.ere pl;iceci on MS containing 5 pM
ABA and 10 1iM GA: and the pl:itcs were pl,icctl nt 4 OC tbr 1. 3. 5. 7 2nd 9 hours on frcsh
~iicdiu~ii \\ it11 5d11ie coiilpo~~c~i~s.
Heo! .\/rock. The expl;~l~tbcar111g c11lbr)oz \vcrc pl;iccd o~i 1111. II~~~I~III~
;12 I~ICIII~OIIC~
iibove ;111d \\SI.C IIICLI~;IIC~ :I\ scl);~s:~te O,IICI~CI ,II 40 'C' :ICI 5U 'C' li)r 30. (10. 9U ;111d 120
IIIIIILI~CI
fiill~\\i'~i by 111~1s II.;III~~;.I 10 fi.esI1 II~C~ILIIII iil~d IIICII~~IIIOI~ 111 IC
CLI~III~~ 1.00111.
Type ut' cu[)l:1111 i, cl.i~cl;rl Il~clor lcir tile :icl~ic\c~lic~lt ol au~t,~blc ~rcgcllcl.;ilio~i ibr
gc11e11e ir~~~isl~r~~i,~t~u~i
~Y]>c~II~~~III>
and proccsslllg cond~t~ons were P~CI);ISC~
\',I~I~LI~
~XIII;II~I~ IYo~ii >cc(ll~~ig~
01 il111k1e111 ,igc, c~~lturt
;iltriiir.c o~rbl.i o ir.uii Thc scctls \vc~c ~oahed ui.cr~ugl~[ ‘inti llic sccil cui~t iraa rclnovcd
rile follo\+,~lig nior11111p 'Tile cutylcdo~ir \vcsc j)I11 opeli :111d the cll~bryo :IAI\ W;IS silrgically
exc~sed ;ind ci~l~urcd OII 111~1li1pli: IUOI
II~~LIC~IUII lilcd~~~~ii tlii11 Ilrid hlS iv~lli BAP wltlliti il
mnge of 5 lu IOU pM III co111b11!;111ufl \vllh ? ul 5 jlL1 klllcl~ii.
Sirool tip Tile dc-coaletl seed> \rere pcsl~i~~i;~lcd un L1S fur 2 dtiys, [he slloot tip (2
mm) \\as tiie~i cxclscd susgicolly 2nd culti~rcd on 111c shoot III~U~IIOII
~ni~diulii (SIM) that
co~lslsted of hlS UIIII 4 pV TDZ, IU KM 2-IP and ? ubl kinellli
56