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3.3 Histological studies oa multiple sltoot ii~itiatio~~ I'I.OIII Ah14 e\pli~~tt:
Preparation of Ahl4 cuplant 1s described 111 tile srctio~~ 3.2.2.1. The liistulogical
studlea \\ere carried out ti.oni llic z~;~gc ~~llcil the ax~ll,ll.y bud \\;15
~ciiloved. Tlic clay of
a\illai) bud rctiiu\;il \\:\a
co~is~dercd ;IS d~y- I The dilielopii~e~i~ ~1'hl\ll4 c\l)I;ltit \.,IS
st~idied
111)to Y days ;ilier the rc~~lo\.~l oS;ixill;~s) b~lcl i.c . 111) IU d,i!-X
Pre/~iii'~~iou. linrriotr iiiid ileln~~irociu~~ ul ii,\\i~r, .\,i~rrp/c.s'Tile c\pIa~llr \vcrc ~prcp.lrud
,111d buli)re !i\3tio11 CUI~IC~OII
\\JS re~lioied D:I\J/ IIS~IIC of IC r~gc~icl.;~ti~ig arc.1 \\,la ,iIau
rc~nuicd ni'ikl~ig 1l1c 5;11nple Into ;I bluck of1 to 5 II~III bide. TIIC 'i~ilpli' \.,IS
~~i~t~~cd~;itely
plilccd In 1ix;hiie so1~1tlo11 (see ilppc~iiii~ Ibi. co~ilpus~t~ul~) i111d ~~osccl OVCIIII~I~I ill 4 'C', l'he
IisaIiic u;I:, d~sc;lrded ,111d IC s;~lllpIcb \\e~.e del~ydr'ilcd \$llli IO"',i, 30U'~i. 4006, 50%. ;1111170%
alcohol scq~lentl:~lly for % Iiui~r c;icli and 1iii;illy 111~) wcle storeil 11170% ;~lcul~ul.
Tlir S~CCIIII~II
\r;l m;lrkctl \villi ;III ~clc~nil)i~ig nllilibcr 1'111s ~iuiiibcs W~IS kept wit11
1Iie t~ssire block tlirouglioul procv\lng. Tlic li,we bloclc i\ilb co~ivcycd IIIIOLI~II
a acrio of
Ibllo\r ing aolients :I, pcs tlic sclicdulc Ibs ileliytl~,~~iu~i, clu,lrlng ;III~I p;~s;ll'li~~ ~i~liltr;~tioii.