April - June 2007 - Kasetsart University

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(Figure 5B). Their viability was 87.14 % and 82.76
% for the four- and six-week-old leaves,
respectively, as determined by FDA staining
(Figure 5C). Protoplast viability decreased
Yield (×10 5 prtoplasts/ g FW)
100
90
80
70
60
50
40
30
20
10
0
a
a
d
c
a
E1 E2 E3
Enzyme mixtures
E4 E5
ab
Yield Viability
Figure 1 Effect of different enzyme mixtures on
yield and viability of C. wendtii
protoplasts. Data represent mean ±
standard error of three replicates.
Yield (×10 5 prtoplasts/ g FW)
120
100
80
60
40
20
0
a
b
c
b
c
c
ab
3 4 5 6
Incubation time (hours)
Yield Viability
Figure 3 Effect of incubation time on yield and
viability of C. wendtii protoplasts.
Data represent mean ± standard error
of three replicates.
c
b
b
Kasetsart J. (Nat. Sci.) 41(2)
a
bc
100
90
80
70
60
50
40
30
20
10
0
90
80
70
60
50
40
30
20
10
0
Viability (%)
Viability (%)
significantly with the increase in leaf age (Figure
4). It was also found a remarkable number of
raphids when using the leaves as a source of
protoplasts.
Yield (×10 5 prtoplasts/ g FW)
100
90
80
70
60
50
40
30
20
10
0
b
ab
d
c
0.4 0.5 0.6 0.7
Mannitol concentration (M)
Yield Viability
Figure 2 Effect of mannitol concentrations in the
enzyme mixture on yield and viability
of C. wendtii protoplasts. Data
represent mean ± standard error of
three replicates.
Yield (×10 5 prtoplasts/ g FW)
120
100
80
60
40
20
0
c
b
c
ab
c
b
b
ab
4 6 8 10
Leaf age (weeks)
Yield Viability
a
a
a
a
0
90
80
70
60
50
40
30
20
10
0
100
Figure 4 Effect of leaf age on yield and viability
of C. wendtii protoplasts. Data
represent mean ± standard error of three
replicates.
90
80
70
60
50
40
30
20
10
Viability (%)
Viability (%)